CAJ | 학술논문

目的:探讨转录因子GATA4在肝癌细胞中的表达及其在肝癌发生中的作用.方法:将GATA4真核表达载体转染小鼠正常肝细胞,RT-PCR、免疫组化鉴定转染效果,G418筛选稳定表达细胞克隆;进行细胞计数并绘制未转染、空转染、转染GATA4组及肝癌细胞组细胞生长曲线;RT-PCR法比较成纤维细胞(3T3,L1)、GATA4转染肝细胞(BNLG,L2)、未转染肝细胞(BNL,L3)、肝癌细胞株H22细胞(H22,L4)、肝癌细胞株Hepa1-6细胞(Hepa1-6,L5)和小鼠孕13.5 d胎肝细胞(孕13.5d胎肝,L6)的GATA4、胎蛋白转录因子(FTF)和甲胎蛋白(AFP)mRNA的表达.结果:GATA4真核表达载体转染效果较好;小鼠肝癌细胞株高表达GATA4 mRNA而正常肝细胞几乎不表达;GATA4转染组小鼠肝细胞增殖速度明显大干未转染和空转染组,但要小于肝癌细胞组.L1～L6的GATA4、FTF、AFP mRNA表达强度分别为0.04±0.02,0.28±0.03,0.04±0.02,0.26±0.03,0.32±0.04,0.24±0.03;0.04±0.02,0.26±0.02,0.08±0.02,0.22±0.02,0.26±0.02,0.26±0.01;0.04±0.01,0.37±0.03,0.14±0.02,0.50±0.03,0.51±0.03,0.49±0.03.L1、L3与L2、L4、L5、L6比较差异显著(P＜0.01),说明正常肝细胞的GATA4、FTF、AFP mRNA表达明显低于肝癌和胎肝细胞,转染后表达增高.结论:GATA4在肝癌细胞中高表达,并能够促进正常肝细胞增殖,促进FTF、AFP mRNA的表达,可能参与肝癌的发生、发展.
목적:탐토전록인자GATA4재간암세포중적표체급기재간암발생중적작용.방법:장GATA4진핵표체재체전염소서정상간세포,RT-PCR、면역조화감정전염효과,G418사선은정표체세포극륭;진행세포계수병회제미전염、공전염、전염GATA4조급간암세포조세포생장곡선;RT-PCR법비교성섬유세포(3T3,L1)、GATA4전염간세포(BNLG,L2)、미전염간세포(BNL,L3)、간암세포주H22세포(H22,L4)、간암세포주Hepa1-6세포(Hepa1-6,L5)화소서잉13.5 d태간세포(잉13.5d태간,L6)적GATA4、태단백전록인자(FTF)화갑태단백(AFP)mRNA적표체.결과:GATA4진핵표체재체전염효과교호;소서간암세포주고표체GATA4 mRNA이정상간세포궤호불표체;GATA4전염조소서간세포증식속도명현대간미전염화공전염조,단요소우간암세포조.L1～L6적GATA4、FTF、AFP mRNA표체강도분별위0.04±0.02,0.28±0.03,0.04±0.02,0.26±0.03,0.32±0.04,0.24±0.03;0.04±0.02,0.26±0.02,0.08±0.02,0.22±0.02,0.26±0.02,0.26±0.01;0.04±0.01,0.37±0.03,0.14±0.02,0.50±0.03,0.51±0.03,0.49±0.03.L1、L3여L2、L4、L5、L6비교차이현저(P＜0.01),설명정상간세포적GATA4、FTF、AFP mRNA표체명현저우간암화태간세포,전염후표체증고.결론:GATA4재간암세포중고표체,병능구촉진정상간세포증식,촉진FTF、AFP mRNA적표체,가능삼여간암적발생、발전.