CAJ | 학술논문

目的:探讨艾灸调控原发性肝细胞肝癌(HCC)癌前病变细胞凋亡的机制.方法:74只SD大鼠随机分为正常组、模型组和艾灸组,以二乙基亚硝胺(DEN)诱导建立HCC模型.艾灸治疗选用"足三里"麦粒灸,3壮/穴,0.5 mg/壮,1次/日,连续16星期,观测体重、肝、胸腺重量的变化;肝脏组织形态学变化以及γ-GT、GST酶学指标变化;采用免疫组化等检测方法观察相关凋亡基因C-myc、N-ras、突变型P53变化趋势及对细胞周期调控基因cyclinD1、CDK4、p16等的影响.结果:艾灸能较明显降低γ-GT和GST在血中的活性;能较为明显减少相关凋亡基因C-myc、N-ras、突变型p53的蛋白表达;能较为明显抑制相关细胞周期调控基因cyclinD1、CDK4过度表达和细胞周期调控基因p16的突变.结论:艾灸可能对减缓HCC癌前病变有一定作用,其作用机制可能与调控部分凋亡基因有关.
목적:탐토애구조공원발성간세포간암(HCC)암전병변세포조망적궤제.방법:74지SD대서수궤분위정상조、모형조화애구조,이이을기아초알(DEN)유도건립HCC모형.애구치료선용"족삼리"맥립구,3장/혈,0.5 mg/장,1차/일,련속16성기,관측체중、간、흉선중량적변화;간장조직형태학변화이급γ-GT、GST매학지표변화;채용면역조화등검측방법관찰상관조망기인C-myc、N-ras、돌변형P53변화추세급대세포주기조공기인cyclinD1、CDK4、p16등적영향.결과:애구능교명현강저γ-GT화GST재혈중적활성;능교위명현감소상관조망기인C-myc、N-ras、돌변형p53적단백표체;능교위명현억제상관세포주기조공기인cyclinD1、CDK4과도표체화세포주기조공기인p16적돌변.결론:애구가능대감완HCC암전병변유일정작용,기작용궤제가능여조공부분조망기인유관.
To investigate the mechanism of moxibustion in regulating cellular apoptosis in rat's precancerous lesion of primary hepatocellular carcinoma (HCC). Methods:Seventy-four rats were randomly allocated to normal group,model group and moxibustion group,and the diethylic nitrosamine (DEN) was used to establish HCC model. Moxibustion with moxa cone which is as big as a grain of wheat was performed on acupoint Zusanli (ST 36),3 cones for each acupoint and 0.5 mg for each cone,the treatment was given once a day,totally 16 weeks. Then the changes in the body weight,liver weight and thymus weight,a morphological change in the liver tissue and changes in γ-GT and GST were observed;Immunohistochemical staining method was adopted to observe the tendency of changes in relevant apoptosis genes such as C-myc,N-ras and mutant type P53,and the influence of moxibustion on cell cycle modulation genes such as cyclinD1,CDK4 and p16. Results:Moxibustion could reduce the activities of γ-GT and GST in the blood,obviously decrease the protein expression of relevant apoptosis genes such as C-myc,N-ras and mutant type P53 and markedly inhibit the over-expression of relevant cell cycle modulation genes such as cyclinD1 and CDK4 and the mutation of cell cycle modulation gene p16. Conclusion:Moxibustion might play a certain role in relieving HCC precancerous lesion and its action mechanism might be related to the regulation on partial apoptosis genes.