CAJ | 학술논문

阿拉伯糖是果实软化过程中变化最明显的细胞壁糖残基之一,α-L-阿拉伯呋喃糖苷酶是导致细胞壁多糖中阿拉伯糖残基降解的主要糖苷酶.为阐明该酶在香蕉果实成熟软化中的作用,实验对香蕉贮藏过程中果皮和果肉中该酶活性以及果实硬度、呼吸强度和乙烯释放量的变化进行了研究.结果表明:α-L-阿拉伯呋喃糖苷酶在果实初期的变化很小,到果实硬度开始急剧下降时达到最大,增加量达10倍以上,且果肉中的酶活性大于果皮中;乙烯吸收剂处理延缓了香蕉果实呼吸和乙烯高峰的出现时间,降低了果实硬度、果皮和果肉中α-L-阿拉伯呋喃糖苷酶活性变化的速度和幅度.以上结果表明α-L-阿拉伯呋喃糖苷酶起诱导香蕉果实成熟的作用,在果实的软化中起着十分重要的作用,且其活性受乙烯的调节.
아랍백당시과실연화과정중변화최명현적세포벽당잔기지일,α-L-아랍백부남당감매시도치세포벽다당중아랍백당잔기강해적주요당감매.위천명해매재향초과실성숙연화중적작용,실험대향초저장과정중과피화과육중해매활성이급과실경도、호흡강도화을희석방량적변화진행료연구.결과표명:α-L-아랍백부남당감매재과실초기적변화흔소,도과실경도개시급극하강시체도최대,증가량체10배이상,차과육중적매활성대우과피중;을희흡수제처리연완료향초과실호흡화을희고봉적출현시간,강저료과실경도、과피화과육중α-L-아랍백부남당감매활성변화적속도화폭도.이상결과표명α-L-아랍백부남당감매기유도향초과실성숙적작용,재과실적연화중기착십분중요적작용,차기활성수을희적조절.
Arabinose is one of the most dynamic cell wall glycosyl residues released during fruit ripening.α-Larabinofuranosidase(α-Arab)are major glycosidases that may remove arabinose units from fruit cell wall polysaccharides.To find out whether α-Arab plays importam roles in banana fruit softening,the enzyme activities in peel and pulp,fruit firmness,respiration rate and ethylene release rate were assayed dunng banana softening.The results showed that α-Arab activities in banana pulp and peel increased slightly at the beginning of storage and reached their maxima when the fruit firmness decreased drastically.α-Arab activity increased by more than ten folds in both pulp and peel during ripening and α-Arab activities were higher in pulp than in peel.Treatment of banana fruits with ethylene absorbent postponed the time of reaching of its maxima of respiration and ethylene,enhanced the firmness of pup and decreased α-Arab activity in the peel and pulp.These results suggest that α-Arab induced the decrease of fruit firmness and played an important role in banana fruit softening,and its activity is regulated by ethylene.