CAJ | 학술논문

背景与目的已有的研究表明,肝素酶与Ki-67抗原与肺癌的发生和转移有关,本研究通过检测非小细胞肺癌(Non-small cell lung cancer,NSCLC)组织中肝素酶(heparanase,Hpa)和Ki-67的表达,以探讨他们的临床意义及相互关系.方法采用免疫组化方法 (S-ABC法和S-P法)检测70例NSCLC和20例同期良性病变肺组织中肝素酶和Ki-67的表达.结果肝素酶和Ki-67的阳性表达率分别为72.9%和85.7%,而正常肺组织中两者均无表达;肝素酶表达水平与患者TNM分期及淋巴结转移有关(P=0.044,P=0.001);Ki-67的表达水平与原发肿瘤大小、组织学类型、癌细胞分化程度、淋巴结转移及TNM分期有关(P<0.05);肝素酶和Ki-67的表达差异无统计学意义(P=0.323).结论肝素酶和Ki-67参与了肺癌的发生和发展,并与预后相关;而肝素酶和Ki-67的表达无相关性,两者可能通过不同的机制参与了肿瘤的发生和发展.
배경여목적 이유적연구표명,간소매여Ki-67항원여폐암적발생화전이유관,본연구통과검측비소세포폐암(Non-small cell lung cancer,NSCLC)조직중간소매(heparanase,Hpa)화Ki-67적표체,이탐토타문적림상의의급상호관계.방법 채용면역조화방법 (S-ABC법화S-P법)검측70례NSCLC화20례동기량성병변폐조직중간소매화Ki-67적표체.결과 간소매화Ki-67적양성표체솔분별위72.9%화85.7%,이정상폐조직중량자균무표체;간소매표체수평여환자TNM분기급림파결전이유관(P=0.044,P=0.001);Ki-67적표체수평여원발종류대소、조직학류형、암세포분화정도、림파결전이급TNM분기유관(P<0.05);간소매화Ki-67적표체차이무통계학의의(P=0.323).결론 간소매화Ki-67삼여료폐암적발생화발전,병여예후상관;이간소매화Ki-67적표체무상관성,량자가능통과불동적궤제삼여료종류적발생화발전.
Background and objective It has been proven that heparanase and Ki-67 had relationship with occur-rence and metastasis in lung cancer. The aim of this work was to investigate the expressions of heparanase and Ki-67 and to ex-plore their clinical significance and mutual relations in non-small cell lung cancer (NSCLC). Methods Immunohistochemical method was used to detect the heparanase and Ki-67expression in the routinely paraffin-embedded sections of surgical specimen of 70 cases with NSCLC and 20 ones with normal lung tissues. Results Heparanase and Ki-67 were highly expressed in lung cancer tissues (72.996 and 85.796) while negative in epithelia of normal lung tissues. The expression of heparanase was signifi-cantly correlated with TNM stage (P=0.044) and lymphatic metastasis (P=0.001). The expression of Ki-67 was significantly cor-related with tumor size (P=0.03) and histology type (P=0.001) and differentiation (P=0.01) and lymphatic metastasis (P=0.01) and TNM stage (P=0.043). The expression ofhaparanase and Ki-67 was no significant difference (P=0.323). Conclusion Hepa-ranase and Ki-67 were involved in the occurrence and development of lung cancer and they may predict patients prognosis, while the expression of heparanase and Ki-67 had no association with NSCLC, which may contribute to the different mechanisms involved in tumor occurrence and development.