CAJ | 학술논문

目的探讨在四氯化碳(CCl4) 复合因素诱导实验性肝硬化大鼠的形成过程中,白介素18(IL-18)、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)的变化及意义.方法将80只清洁级雄性SD大鼠随机分为正常对照组和造模2周组、4周组、6周组,每组20只.予以CCl4复合因素诱导大鼠肝硬化,并在2、4、6周3个时间点分别处死6只大鼠.采用ELISA法检测大鼠血清IL-18、TNF-α、IFN-γ和肝脏组织匀浆上清液中的IL-18含量;HE染色观察肝脏的组织病理学变化.结果 ①CCl_4复合因素诱导的实验性大鼠,组织病理学观察发现,2周时大鼠肝脏组织细胞肿胀变性,6周时有大量纤维增生,部分肝组织有假小叶的形成;②随着造模时间的延长,实验大鼠血清IL-18、TNF-α、IFN-γ水平逐渐升高,造模6周组与正常对照组比较差异有统计学意义(P<0.01);③造模组肝脏组织匀浆中IL-18随肝损害程度的加重而升高,造模6周组与正常对照组比较差异有统计学意义(P<0.01).结论在CCl_4复合因素诱导大鼠肝硬化的形成过程中,IL-18、TNF-α、IFN-γ水平逐渐升高,提示该3种细胞因子与肝硬化的形成及发展有关.
목적 탐토재사록화탄(CCl4) 복합인소유도실험성간경화대서적형성과정중,백개소18(IL-18)、종류배사인자α(TNF-α)、γ간우소(IFN-γ)적변화급의의.방법 장80지청길급웅성SD대서수궤분위정상대조조화조모2주조、4주조、6주조,매조20지.여이CCl4복합인소유도대서간경화,병재2、4、6주3개시간점분별처사6지대서.채용ELISA법검측대서혈청IL-18、TNF-α、IFN-γ화간장조직균장상청액중적IL-18함량;HE염색관찰간장적조직병이학변화.결과 ①CCl_4복합인소유도적실험성대서,조직병이학관찰발현,2주시대서간장조직세포종창변성,6주시유대량섬유증생,부분간조직유가소협적형성;②수착조모시간적연장,실험대서혈청IL-18、TNF-α、IFN-γ수평축점승고,조모6주조여정상대조조비교차이유통계학의의(P<0.01);③조모조간장조직균장중IL-18수간손해정도적가중이승고,조모6주조여정상대조조비교차이유통계학의의(P<0.01).결론 재CCl_4복합인소유도대서간경화적형성과정중,IL-18、TNF-α、IFN-γ수평축점승고,제시해3충세포인자여간경화적형성급발전유관.
Objective To investigate the changes of interleukin-18 (IL-18), tumor necrosis factor-α(TNF-α) and interferon-γ (IFN-γ) levels of liver cirrhosis induced by the composite factors of carbon tetrachloride (CCl_4) in SD rats and their significance. Methods Totally 80 male SD rats of clean class were randomly divided into normal control group (20 rats) and model groups, the latter of which were further divided into three groups according to the length of administration time, namely, 2-week group (2 wk group), 4-week group (4 wk group) and 6-week group (6 wk group), with 20 rats in each. Six rats were killed after 2 wk, 4 wk and 6 wk administration time, respectively. The rat serum levels of IL-18, TNF-α and IFN-γ and the hepatic homogenate supernatant of IL-18 were detected by ELISA; pathological changes of liver tissues were observed by HE staining. Results ① Pathological observation revealed that in the model groups hepatic cells degenerated and swelled at week 2 while large amounts of fibrosis and pseudolobules of some liver tissues occurred at week 6. ② The serum levels of IL-18, TNF-α and IFN-γ were gradually increased with the modeling time, and they were significantly higher in 6-week group than in normal control group (P<0.01). ③ The levels of hepatic homogenate supernatant of IL-18 in the model groups were elevated with liver damage, and they were significantly higher in 6-week group than in normal control group (P<0.01). Conclusion During the formation of liver cirrhosis induced by composite factors of CCl_4 in rats, IL-18, TNF-α and IFN-γ levels gradually increase, suggesting that the three cytokines play a certain role during the occurrence of liver cirrhosis in rats.