CAJ | 학술논문

目的探讨大鼠脊髓源神经干细胞与运动神经元的差异蛋白质组学,寻找出重要的差异蛋白质.方法采用双向电泳分离两种细胞的蛋白质,用DeCyder软件分析蛋白表达差异,并采用质谱(HPLC-ESI-MS/MS)进行鉴定.结果脊髓源神经干细胞与运动神经元蛋白质凝胶分析获得1 300余个清晰的蛋白质斑点,在比国产分析的87个差异点中,初步鉴定出44个差异表达蛋白,其中24个在神经干细胞高表达,20个在运动神经元高表达.结论脊髓源神经干细胞与运动神经元有各自特定的蛋白质表达,某些差异蛋白可能在神经干细胞向运动神经元分化中发挥关键作用.
목적 탐토대서척수원신경간세포여운동신경원적차이단백질조학,심조출중요적차이단백질.방법 채용쌍향전영분리량충세포적단백질,용DeCyder연건분석단백표체차이,병채용질보(HPLC-ESI-MS/MS)진행감정.결과 척수원신경간세포여운동신경원단백질응효분석획득1 300여개청석적단백질반점,재비국산분석적87개차이점중,초보감정출44개차이표체단백,기중24개재신경간세포고표체,20개재운동신경원고표체.결론 척수원신경간세포여운동신경원유각자특정적단백질표체,모사차이단백가능재신경간세포향운동신경원분화중발휘관건작용.
Objective To analysis and compare the proteomic differences between neural stem cells and motor neurons in embryonic spinal cord in rat and discover the key different proteins. Methods Separating the protein of cells by the 2-D fluorescence difference gel electrophoresis, and to analyse the differences of protein expression with DeCyder software, and to identify with high performance liquid chromatography-electrospray tandem mass spectrometry. Results About 1 300 protein spots from the cells were gained after gel analysis. Eighty-seven protein spots were selected for mass spectrometry analysis. Fourty-four differently expressed proteins (24 in neural stem cells and 20 in motor neurons) were identified by mass spectrometry analysis.Conclusion Differently expressed proteins between neural stem cells and motor neurons were identified and it is helpful to find the new targets in neural stem cells differentiation into motor neurons.