CAJ | 학술논문

目的观察体外巨噬细胞对视网膜神经节细胞(RGC)存活和生长的影响.方法采用Transwell小室建立大鼠腹膜腔巨噬细胞和RGC的共培养模型,以不用巨噬细胞共培养的RCC作为对照组,用台盼蓝染色计数及相差显微镜观察RGC存活的时间,并测量培养1 d、3 d和5 d时RGC突起的平均长度,进行组间比较.结果培养1 d、3 d、5 d和7 d,共培养组平均活细胞数分别为(35.50±2.92)个、(28.20±3.36)个、(18.70±3.95)个和(8.80±1.55)个,对照组为(36.20±2.35)个、(27.10±2.96)个、(15.80±3.04)个和(8.40±2.01)个,两组之间差异均无统计学意义(t=0.369、0.497、0.487、2.854,P均>0.05).培养1 d、3 d和5 d,共培养组RGC突起的平均长度分别为(19.79±3.98)μm、(68.30±4.07)μm和(95.51±6.51)μm,明显长于对照组[(15.28±1.28)μm、(58.18±4.22)μm和(82.61±3.75)μm],两组差异均有统计学意义(t=-4.562、-6.554、-7.027,P均<0.05).结论巨噬细胞可明显促进共培养的RGC轴突的生长,但对其存活时间没有显著影响.
목적 관찰체외거서세포대시망막신경절세포(RGC)존활화생장적영향.방법 채용Transwell소실건립대서복막강거서세포화RGC적공배양모형,이불용거서세포공배양적RCC작위대조조,용태반람염색계수급상차현미경관찰RGC존활적시간,병측량배양1 d、3 d화5 d시RGC돌기적평균장도,진행조간비교.결과 배양1 d、3 d、5 d화7 d,공배양조평균활세포수분별위(35.50±2.92)개、(28.20±3.36)개、(18.70±3.95)개화(8.80±1.55)개,대조조위(36.20±2.35)개、(27.10±2.96)개、(15.80±3.04)개화(8.40±2.01)개,량조지간차이균무통계학의의(t=0.369、0.497、0.487、2.854,P균>0.05).배양1 d、3 d화5 d,공배양조RGC돌기적평균장도분별위(19.79±3.98)μm、(68.30±4.07)μm화(95.51±6.51)μm,명현장우대조조[(15.28±1.28)μm、(58.18±4.22)μm화(82.61±3.75)μm],량조차이균유통계학의의(t=-4.562、-6.554、-7.027,P균<0.05).결론 거서세포가명현촉진공배양적RGC축돌적생장,단대기존활시간몰유현저영향.
Objective To determine the effects of macrophages on the survival and axonal regeneration of retinal ganglion cells (RGC) in vitro. Methods A rat co -culture model was established with RGC and peritoneal cavity macrophages in transwell chambers. RGC cultured without macrophages served as the control. The axonal growth and survival time of RGC in the model were observed under phase contrast microscopy. The survival of RGC was determined by counting the cells with Trypan blue staining. The average lengths of the processes of RGC cultured on days 1, 3 and 5 were measured and calculated. Results The average counts of viable cells with Trypan blue staining in the co-culture system were 35.50±2.92, 28.20±3.36, 18.70±3.95, and 8.80±1.55 on days 1, 3, 5 and 7, respectively. And there were no statistically significant differences when compared to the controls (36.20±2.35, 27.10±2.96, 15.80±3.04, and 8.40±2.01, respectively;t=0.369, 0.497, 0.487, and 2.854; P>0.05). The average lengths of the RGC processes in the co-culture system were (19.79±3.98)μm, (68.30±4.07)μn, and (95.51 ±6.5l)μm on days 1, 3 and 5, respectively, which were significantly longer when compared to the controls [(15.28±1.28)μm, (58.18±4.22)μm, and (82.61 ±3.75)μm, respectively; t =-4.562, -6.554, -7.027; P<0.05). Conclusion Results shows that macrophages could significantly promote the axonal regeneration of RGC in a co-culture system, but there is no obvious effect on cell survival.