CAJ | 학술논문

目的观察早期应用冬眠合剂对严重烫伤大鼠肺损伤是否具有保护作用. 方法建立Ⅲ度烫伤大鼠模型,随机分为冬眠合剂组和对照组(未用冬眠合剂),每组36只.分别在伤后3、5、7、10 d测定大鼠动脉血氧分压,检测肺组织中过氧化物酶(MPO)活性、丙二醛(MDA)含量、肿瘤坏死因子α(TNF-α)及γ干扰素(IFN-γ)蛋白表达,动态观察伤后肺组织病理学变化. 结果与对照组大鼠伤后3 d动脉血氧分压(8.86±0.23)kPa(1 kPa=7.5 mm Hg)比较,冬眠合剂组该时相点明显升高[(12.58±0.41)kPa,P<0.01].冬眠合剂组大鼠伤后各时相点肺组织MPO活性和MDA含量下降(P<0.05或P<0.01);伤后3、5、7 d肺组织TNF-α表达显著下调(P<0.05或P<0.01),伤后5、7、10 d肺组织IFN-γ的表达亦显著下调(P<0.01).冬眠合剂组大鼠肺泡间质水肿减轻,炎性细胞浸润减少. 结论大鼠烫伤后及时复苏并给予冬眠合剂,能适度抑制机体应激反应并下调促炎因子表达,改善早期肺功能.
목적 관찰조기응용동면합제대엄중탕상대서폐손상시부구유보호작용. 방법건립Ⅲ도탕상대서모형,수궤분위동면합제조화대조조(미용동면합제),매조36지.분별재상후3、5、7、10 d측정대서동맥혈양분압,검측폐조직중과양화물매(MPO)활성、병이철(MDA)함량、종류배사인자α(TNF-α)급γ간우소(IFN-γ)단백표체,동태관찰상후폐조직병이학변화. 결과여대조조대서상후3 d동맥혈양분압(8.86±0.23)kPa(1 kPa=7.5 mm Hg)비교,동면합제조해시상점명현승고[(12.58±0.41)kPa,P<0.01].동면합제조대서상후각시상점폐조직MPO활성화MDA함량하강(P<0.05혹P<0.01);상후3、5、7 d폐조직TNF-α표체현저하조(P<0.05혹P<0.01),상후5、7、10 d폐조직IFN-γ적표체역현저하조(P<0.01).동면합제조대서폐포간질수종감경,염성세포침윤감소. 결론대서탕상후급시복소병급여동면합제,능괄도억제궤체응격반응병하조촉염인자표체,개선조기폐공능.
Objective To investigate the protective effect of inhibition of stress (lytic cocktail) on lung injury in severe bum rats at early stage. Methods Sprague-Dawley rats inflicted with 30% TBSA full- thickness burn were randomly divided into A group ( n = 36, fluid resuscitation with administration of lyric cocktail) , B group( n = 36, fluid resuscitation only). Lung function was evaluated by partial pressure of ox- ygen ( PaO2 ) in arterial blood and histopathologic changes on 3, 5, 7, 10 post bum day (PBD). The levels of malonyldialdehyde (MDA) , myeloperoxidase (MPO) , tumor necrosis factor-α(TNF-α) and interferon-γ (IFN-γ) in lung tissue were measured at the same time points. Results The PaO2 level in A group on 3 PBD (12.58±0.41 kPa) was significantly higher than that in B group ( 8.86±0. 23 kPa, P < 0.01 ). Compared with those in B group, the levels of MDA and MPO were significantly decreased in A group at each time point( P <0.05 or0.01) ,the levels of TNF-α on 3,5,7 PBD ( P <0.05 or0.01)and IFN-γ on 5,7,14 PBD( P < 0. 01 ) were also decreased in A group. Swollen lung mesenchyme was alleviated,infiltra- tion of inflammatory cell was lessened in A group. Conclusion Lytic cocktail combined with immediate fluid resusitation can inhibit stess response, downregulate the expression of inflammatory factor, ameliorate lung function in severe burn rat at early stage.