中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
2期
151-155
,共5页
白慧玲%王雪银%李淑莲%黄红莹%杜耀武%刘广超%马远方
白慧玲%王雪銀%李淑蓮%黃紅瑩%杜耀武%劉廣超%馬遠方
백혜령%왕설은%리숙련%황홍형%두요무%류엄초%마원방
内毒素%脂多糖%可溶性死亡受体5%肿瘤坏死因子相关凋亡诱导配体%肝细胞凋亡
內毒素%脂多糖%可溶性死亡受體5%腫瘤壞死因子相關凋亡誘導配體%肝細胞凋亡
내독소%지다당%가용성사망수체5%종류배사인자상관조망유도배체%간세포조망
Endotoxin%Lipopolysaccharides%Soluble death receptor 5%TNF related apoptosis inducing ligand%Hepatocyte apoptosis
目的 探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)在内毒素诱导小鼠肝细胞凋亡中的作用.方法 内毒素作用于可溶性死亡受体-5(sDR5)封闭TRAIL的小鼠,不同时间检测小鼠血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)和乳酸脱氧酶(LDH)的含量;HE染色及Annexin V、PI双染法流式细胞术检测小鼠肝细胞凋亡;免疫组织化学法及流式细胞术检测小鼠肝细胞和肝细胞膜表面DR5的表达.结果 内毒素可以上调肝细胞DR5的表达.DR5封闭TRAIL可显著抑制内毒素引起的小鼠肝细胞凋亡和改善肝细胞损伤.结论 内毒素引起疾病过程中TRAIL起重要作用.
目的 探討腫瘤壞死因子相關凋亡誘導配體(TRAIL)在內毒素誘導小鼠肝細胞凋亡中的作用.方法 內毒素作用于可溶性死亡受體-5(sDR5)封閉TRAIL的小鼠,不同時間檢測小鼠血清中的穀丙轉氨酶(ALT)、穀草轉氨酶(AST)和乳痠脫氧酶(LDH)的含量;HE染色及Annexin V、PI雙染法流式細胞術檢測小鼠肝細胞凋亡;免疫組織化學法及流式細胞術檢測小鼠肝細胞和肝細胞膜錶麵DR5的錶達.結果 內毒素可以上調肝細胞DR5的錶達.DR5封閉TRAIL可顯著抑製內毒素引起的小鼠肝細胞凋亡和改善肝細胞損傷.結論 內毒素引起疾病過程中TRAIL起重要作用.
목적 탐토종류배사인자상관조망유도배체(TRAIL)재내독소유도소서간세포조망중적작용.방법 내독소작용우가용성사망수체-5(sDR5)봉폐TRAIL적소서,불동시간검측소서혈청중적곡병전안매(ALT)、곡초전안매(AST)화유산탈양매(LDH)적함량;HE염색급Annexin V、PI쌍염법류식세포술검측소서간세포조망;면역조직화학법급류식세포술검측소서간세포화간세포막표면DR5적표체.결과 내독소가이상조간세포DR5적표체.DR5봉폐TRAIL가현저억제내독소인기적소서간세포조망화개선간세포손상.결론 내독소인기질병과정중TRAIL기중요작용.
Objective To explore the effect of TNF related apoptosis inducing ligand (TRAIL) in apoptosis induced by LPS. Methods After LPS injected mice blocking TRAIL with soluble death receptor 5 (sDRS), detecting ALT, AST and LDH of mice serum at different times, apoptotic effects of LPS to mice hepatocyte were detected by HE and flow eytometry (FCM) with Annexin V-FITC/PI staining. The expres-sion of DR5 in mice hepatocyte was assayed with immunohistochemistry and FCM. Results Apoptotic effect was promoted by up-regulated DR5 expression on hepatocyte. Blocking TRAIL with sDR5 markedly amelio-rated the hepatocyte damage and reduced apoptosis. Conclusion These results establish a critical role for TRAIL in apoptosis during disease process of LPS.
