中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2010年
11期
8-11
,共4页
银屑病%T淋巴细胞%CD134(OX40)
銀屑病%T淋巴細胞%CD134(OX40)
은설병%T림파세포%CD134(OX40)
Psoriasis%T lymphocytes%CD134(OX40)
目的 了解银屑病患者外周血T细胞CD69和CD134的表达情况及多克隆刺激剂对其表达的影响.方法 取银屑病患者与健康志愿者外周血全血培养,佛波醇脂(PMA)联合Ionomycin刺激使T细胞体外活化;在刺激第0、6、12、24 h,用三色免疫荧光标记流式细胞术检测T细胞CD69、CD134的表达.结果 银屑病患者组和健康对照组各11例纳入研究,结果显示:①银屑病患者组新鲜血标本T细胞CD134、CD69表达率均明显高于健康对照组(P<0.01); ②PMA联合Ionomycin刺激第6、12、24 h,银屑病患者组与健康对照组外周血T细胞CD69表达率仅在第24 h差异具有统计学意义(P<0.05); ③在刺激第6、12、24 h,银屑病患者组外周血T细胞CD134表达率均较健康对照组显著升高(P<0.001).结论 银屑病患者外周血T细胞存在异常活化.在体外活化的外周血T细胞上,银屑病患者组CD134的表达显著高于健康对照组.
目的 瞭解銀屑病患者外週血T細胞CD69和CD134的錶達情況及多剋隆刺激劑對其錶達的影響.方法 取銀屑病患者與健康誌願者外週血全血培養,彿波醇脂(PMA)聯閤Ionomycin刺激使T細胞體外活化;在刺激第0、6、12、24 h,用三色免疫熒光標記流式細胞術檢測T細胞CD69、CD134的錶達.結果 銀屑病患者組和健康對照組各11例納入研究,結果顯示:①銀屑病患者組新鮮血標本T細胞CD134、CD69錶達率均明顯高于健康對照組(P<0.01); ②PMA聯閤Ionomycin刺激第6、12、24 h,銀屑病患者組與健康對照組外週血T細胞CD69錶達率僅在第24 h差異具有統計學意義(P<0.05); ③在刺激第6、12、24 h,銀屑病患者組外週血T細胞CD134錶達率均較健康對照組顯著升高(P<0.001).結論 銀屑病患者外週血T細胞存在異常活化.在體外活化的外週血T細胞上,銀屑病患者組CD134的錶達顯著高于健康對照組.
목적 료해은설병환자외주혈T세포CD69화CD134적표체정황급다극륭자격제대기표체적영향.방법 취은설병환자여건강지원자외주혈전혈배양,불파순지(PMA)연합Ionomycin자격사T세포체외활화;재자격제0、6、12、24 h,용삼색면역형광표기류식세포술검측T세포CD69、CD134적표체.결과 은설병환자조화건강대조조각11례납입연구,결과현시:①은설병환자조신선혈표본T세포CD134、CD69표체솔균명현고우건강대조조(P<0.01); ②PMA연합Ionomycin자격제6、12、24 h,은설병환자조여건강대조조외주혈T세포CD69표체솔부재제24 h차이구유통계학의의(P<0.05); ③재자격제6、12、24 h,은설병환자조외주혈T세포CD134표체솔균교건강대조조현저승고(P<0.001).결론 은설병환자외주혈T세포존재이상활화.재체외활화적외주혈T세포상,은설병환자조CD134적표체현저고우건강대조조.
Objective To investigate the in vivo expression of CD134 by the peripheral blood T cells from psoriasis patients, the regulation of their expression in response to polyclone stimulator. Methods The whole blood culture procedure was applied to mimic the natural conditions of T cell activation in vivo. T cells were activated by polyclone stimulator PMA plus Ionomycine. CD69 and CD134 expressed in vitro on activated T lymphocytes from psoriasis patients and healthy controls were detected with three-color flow cytometry. Results The results showed that the expression rates of CD134 and CD69 on the peripheral T cells from psoriasis patients were much higher than those of healthy controls (P<0.05). In response to the stimulation of PMA plus Ionomycin, there was only significant difference on the expression rates of CD69 on the peripheral blood T cells between these two groups at 24 h post activation. Whereas, the expression rates of CD134 on psoriatic peripheral blood T cells were significantly increased than those of the healthy controls at all time points (P<0.001). Conclusions The results verified the aberrant activation of the peripheral blood T lymphocytes in psoriasis. Moreover, the expression of CD134 on psoriatic peripheral T cells in response to polyclone stimulators PMA plus Ionomycin is in a stronger manner than those of healthy controls.
