CAJ | 학술논문

目的采用Transwell小室筛选胃癌细胞高侵袭亚系并研究其生物学特性.方法利用Transwell小审筛选高侵袭力胃痛细胞亚系,通过HE染色、Western blotting、Transwell小室模型、免疫组织化学法及生长曲线研究亚系的生物学特性.结果利用Transwell小室反复筛选10次,从MKN-28细胞中筛选出高侵袭力胃癌细胞亚系MKN-28S10.母亚两系细胞形态上无明显差异,但亚系中E-cadherin和TIMP-1蛋门表达同母系相比显著降低(P<0.05),显微镜下细胞计数示亚系迁移至膜背面的数量(100.25±0.50/视野)较母系(61.75±2.06/视野)明显增多(P<0.05),细胞运动能力明显增强;免疫组织化学结果显示,亚系中NM23-H1的表达明显低于母系(P<0.05);亚系体外生长速度快于母系.结论 MKN-28S10细胞较MKN-28细胞具有更高侵袭力和更强增生能力.
목적 채용Transwell소실사선위암세포고침습아계병연구기생물학특성.방법 이용Transwell소심사선고침습력위통세포아계,통과HE염색、Western blotting、Transwell소실모형、면역조직화학법급생장곡선연구아계적생물학특성.결과 이용Transwell소실반복사선10차,종MKN-28세포중사선출고침습력위암세포아계MKN-28S10.모아량계세포형태상무명현차이,단아계중E-cadherin화TIMP-1단문표체동모계상비현저강저(P<0.05),현미경하세포계수시아계천이지막배면적수량(100.25±0.50/시야)교모계(61.75±2.06/시야)명현증다(P<0.05),세포운동능력명현증강;면역조직화학결과현시,아계중NM23-H1적표체명현저우모계(P<0.05);아계체외생장속도쾌우모계.결론 MKN-28S10세포교MKN-28세포구유경고침습력화경강증생능력.
Objective To study a gastric carcinoma subcell line with higher invasive potential and its biologic characteristics screened by a transwell chamber. Methods Transwell chamber was used for the selection of tumor subline. The biological characteristics of the cell lines were studied with optics microscope, Westem blotting, Transwell, immunohistochemical staining and growth curve. Results A gastric carcinoma cell subcell line (named MKN-28S10) was established from its parent cell line MKN-28 with higher invasive potential. MKN-28S10 showed essentially the same morphous as MKN-28. The expression of E-cadherin and TIMP-1 decreased significantly in the screened subcell line(P<0.05). The expression of NM23-H1 in MKN-28S10 was significantly lower than that in MKN-28 (P<0.05). Compared with MKN-28 (61.75±2.06 per vision), the migrative ability of passing through the membrane Millipore(100.25±0.50 per vision) was obviously increased in MKN-28S10(P<0.05). Compared with MKN-28, the growth rate of MKN-28S10 was increased obviously. Conclusion MKN-28S10 cell has stronger invasive ability and more powerful proliferation than that of its mother line MKN-28.