CAJ | 학술논문

于2000年2月以中国科学院海洋研究所水族楼暂养的中国对虾为材料，采用常规方法从尾部肌肉中提取DNA，构建小片段部分基因组文库。采用人工设计合成的(CT)7、(AT)7重复片段作引物，利用PCR法对中国对虾小片段部分基因组文库进行筛选。本实验首次在中国对虾中获得31个微卫星序列，分别分布于18个阳性重组克隆中，其中perfect共23个，占74%，imperfect 2个，占7%，compound perfect?1个，占3%，compound imperfect 5个，占16%。结果还表明，在中国对虾基因组DNA中，(CT)n、(AT)n形式的微卫星序列的含量非常丰富。
우2000년2월이중국과학원해양연구소수족루잠양적중국대하위재료，채용상규방법종미부기육중제취DNA，구건소편단부분기인조문고。채용인공설계합성적(CT)7、(AT)7중복편단작인물，이용PCR법대중국대하소편단부분기인조문고진행사선。본실험수차재중국대하중획득31개미위성서렬，분별분포우18개양성중조극륭중，기중perfect공23개，점74%，imperfect 2개，점7%，compound perfect?1개，점3%，compound imperfect 5개，점16%。결과환표명，재중국대하기인조DNA중，(CT)n、(AT)n형식적미위성서렬적함량비상봉부。
Thirty-one microsatellites of the Chinese shrimp Penaeus chinensis were obtained through PCR screening small size fractionated libraries of P. chinensis with simple tandem repeats primers (AT)n and (CT)n. The bacterial suspensions were used in PCR. A high temperature of 95℃ was used for breaking the bacteria. After screening,recombinant positive clones containing microsatellites were sequenced. Among the 31 microsatellites in 18 positive recombinant positive clones are 23 perfect ones (74%),2 imperfect (7%),1 compound perfect (3%),and 5 compound imperfect (16%). The experiment shows that microsatellite sequences characterized by (AT)n and (CT)n abundant in genomic DNA of Chinese shrimp. Primers can be designed according to these microsatellite franking sequences and used in genetic analysis.