CAJ | 학술논문

目的:通过对感染丝裂素活化蛋白激酶(Mitogen-Activated Protein Kinase,MAPK)细胞外信号调节激酶(extracellular signal-regulated kinase包括ERK1和ERK2)的信号通路的抑制作用,研究中药金叶败毒制剂治疗巨细胞病毒(humancytomegalovirus,HCMV)感染的分子机制.方法:使用免疫印记技术检测中药金叶败毒制剂和更昔洛韦(ganciclovir,GCV)干预HCMV感染的人胚肺成纤维细胞(human embryonic lung,HEL)的ERK表达水平,并观察MEK(mitogen-activated protein kinase kinase)特异性抑制剂PD 98059,中药金叶败毒制剂对细胞的病变作用(cytopathic effect,CPE)的影响.结果:两种药物都可抑制HCMV在HEL中的增殖,以PD 98059与中药金叶败毒制剂合用效果明显.中药金叶败毒制剂使磷酸化ERK1/2表达降低,对ERK1的抑制作用在感染后10min出现,30min达到高峰,对ERK2的抑制作用在感染后10min出现,60min达到高峰,而GCV对ERK1/2无明显的抑制作用.结论:中药金叶败毒制剂可通过调节MAPK/ERK通路而抑制HCMV基因的表达和复制从而发挥其部分抗病毒作用.
목적:통과대감염사렬소활화단백격매(Mitogen-Activated Protein Kinase,MAPK)세포외신호조절격매(extracellular signal-regulated kinase포괄ERK1화ERK2)적신호통로적억제작용,연구중약금협패독제제치료거세포병독(humancytomegalovirus,HCMV)감염적분자궤제.방법:사용면역인기기술검측중약금협패독제제화경석락위(ganciclovir,GCV)간예HCMV감염적인배폐성섬유세포(human embryonic lung,HEL)적ERK표체수평,병관찰MEK(mitogen-activated protein kinase kinase)특이성억제제PD 98059,중약금협패독제제대세포적병변작용(cytopathic effect,CPE)적영향.결과:량충약물도가억제HCMV재HEL중적증식,이PD 98059여중약금협패독제제합용효과명현.중약금협패독제제사린산화ERK1/2표체강저,대ERK1적억제작용재감염후10min출현,30min체도고봉,대ERK2적억제작용재감염후10min출현,60min체도고봉,이GCV대ERK1/2무명현적억제작용.결론:중약금협패독제제가통과조절MAPK/ERK통로이억제HCMV기인적표체화복제종이발휘기부분항병독작용.