CAJ | 학술논문

从患腹水病褐牙鲆体内分离致病菌,通过生理生化和分子生物学方法进行鉴定;该菌的16S rDNA序列与标准株同源性达99.8%,证明该菌为嗜水气单胞菌.制备灭活疫苗后分别注射小鼠和牙鲆,测定其免疫效果.试验结果表明,用所制备的灭活疫苗免疫小鼠,其保护率达到90%,免疫牙鲆的保护率达到80%;采用渗透压差法浸泡免疫牙鲆在攻毒后发病率仅为6%,攻毒后保护率为85%;通过间接ELISA检测表明受免动物体内产生了较高水平的抗体;剖检及病理切片结果表明免疫过的动物内脏健康无异样,而未免动物肝脏充血,肠腔有积水.说明该疫苗安全有效.
종환복수병갈아평체내분리치병균,통과생리생화화분자생물학방법진행감정;해균적16S rDNA서렬여표준주동원성체99.8%,증명해균위기수기단포균.제비멸활역묘후분별주사소서화아평,측정기면역효과.시험결과표명,용소제비적멸활역묘면역소서,기보호솔체도90%,면역아평적보호솔체도80%;채용삼투압차법침포면역아평재공독후발병솔부위6%,공독후보호솔위85%;통과간접ELISA검측표명수면동물체내산생료교고수평적항체;부검급병리절편결과표명면역과적동물내장건강무이양,이미면동물간장충혈,장강유적수.설명해역묘안전유효.
The pathogenic bacteria were isolated from diseased Japanese flounder Paralichthys olivaceus, and identified as Aeromonas hydrophila by biochemical methods and polymerase chain reaction based on the 16s rDNA sequence on GenBank via the homologies of nucleic acids of 99.8% between the isolated strain and the standard strains. The mice and the flounder were immunized with the inactivated vaccine made by this isolated Aeromonas hydrophila via dipping immersion and showed protection rate of 90% and 80% with the incidence of 6%. The antibody level was detected by agglutination reaction and indirect enzyme-linked immunosorbent assay (ELISA) with the antiserum seemed immunogenic crucially. The anatomized results showed that the immunized mice were healthy and the unimmunized mice (as the control) were diseased in liver, indicating that the vaccine was effective.