CAJ | 학술논문

以牛ANGPTL4基因为研究对象,运用生物信息学方法对其编码蛋白的结构、理化性质、信号肽、跨膜结构、亚细胞定位、二级结构以及高级结构进行生物信息学分析,并推测与其他物种的生物进化关系.结果表明,牛ANGPTL4蛋白是一个疏水性不稳定蛋白,定位于细胞外.含有12个α-螺旋,8个β-折叠,30个转角,3个跨膜结构区域.通过分子进化树研究发现牛的ANGPTL4基因在人、小鼠、大鼠、猪、黑猩猩、狗、鸡等物种中,与猪的亲缘关系最近.
이우ANGPTL4기인위연구대상,운용생물신식학방법대기편마단백적결구、이화성질、신호태、과막결구、아세포정위、이급결구이급고급결구진행생물신식학분석,병추측여기타물충적생물진화관계.결과표명,우ANGPTL4단백시일개소수성불은정단백,정위우세포외.함유12개α-라선,8개β-절첩,30개전각,3개과막결구구역.통과분자진화수연구발현우적ANGPTL4기인재인、소서、대서、저、흑성성、구、계등물충중,여저적친연관계최근.
ANGPTL4 gene in cattle was investigated.By means of bioinformatics analysis its encoding protein was analyzed including its structure,physico-chemical property,signal peptide,transmembrane structure,subcellular localization,secondary structure and higher structure,and its evolution relationship with other species was inferred.The results indicated that ANGPTL4 protein of cattle was a kind of hydrophobicity labile protein,locating cellular exterior,which contained 12 α-helics,8 β-strands,30 turns and 3 transmembrane domains. It might be concluded that ANGPTL4 gene of cattle has closeet affinity with that of pig,compared with that of human,mus,rat,pan,dog and fowl based on the analysis of molecular evolution.