CAJ | 학술논문

背景:目前所使用的全身振动防治骨质疏松所需振动强度较大,人体不适感较强.作者设计了复合振动,前期实验发现复合振动可在更低强度下有效预防卵巢切除大鼠的骨密度下降. 目的:课题创新性提出低强度复合振动可维持生长期卵巢切除SD大鼠骨质量的理论假设,并期望实验结果加以验证.方法:SPF级4月龄雌性未育SD大鼠32只,随机分为正常对照组、卵巢切除组以及振动1、振动2组,每组大鼠均为8只.振动1组接振45～55Hz,0.05～0.1 g;振动2组接振45～55Hz,0.12～0.21 g.振动20min/次,1次/d,5次/周,休息间隔不大于2d.实验时间13周.观察振动干预前后大鼠活体骨密度,体外标本骨微结构以及生物力学性能. 结果与结论:卵巢切除组腰椎骨密度下降(P<0.05),而正常对照组与两振动组有显著性增加,股骨骨密度均增加,组间差异无显著性意义;卵巢切除各组骨微结构参数均明显下降,但振动2组骨小梁数量、骨小梁厚度、骨小梁间距、骨体积分数相对于卵巢切除组有显著改善:腰椎骨强度值两振动组较卵巢切除组显著增加(P=0.025、0.006),与正常对照组比较差异无显著性意义.实验结果证明,特定的复合振动舒适感较好的低强度下可以有效预防卵巢切除SD大鼠骨密度下降,减轻骨微结构破坏程度,维持骨强度,具有改善卵巢切除大鼠骨质量的作用和潜在的预防骨质疏松作用.
배경:목전소사용적전신진동방치골질소송소수진동강도교대,인체불괄감교강.작자설계료복합진동,전기실험발현복합진동가재경저강도하유효예방란소절제대서적골밀도하강. 목적:과제창신성제출저강도복합진동가유지생장기란소절제SD대서골질량적이론가설,병기망실험결과가이험증.방법:SPF급4월령자성미육SD대서32지,수궤분위정상대조조、란소절제조이급진동1、진동2조,매조대서균위8지.진동1조접진45～55Hz,0.05～0.1 g;진동2조접진45～55Hz,0.12～0.21 g.진동20min/차,1차/d,5차/주,휴식간격불대우2d.실험시간13주.관찰진동간예전후대서활체골밀도,체외표본골미결구이급생물역학성능. 결과여결론:란소절제조요추골밀도하강(P<0.05),이정상대조조여량진동조유현저성증가,고골골밀도균증가,조간차이무현저성의의;란소절제각조골미결구삼수균명현하강,단진동2조골소량수량、골소량후도、골소량간거、골체적분수상대우란소절제조유현저개선:요추골강도치량진동조교란소절제조현저증가(P=0.025、0.006),여정상대조조비교차이무현저성의의.실험결과증명,특정적복합진동서괄감교호적저강도하가이유효예방란소절제SD대서골밀도하강,감경골미결구파배정도,유지골강도,구유개선란소절제대서골질량적작용화잠재적예방골질소송작용.
BACKGROUND: Whole body vibration which is used to prevent and treat osteoporosis recently is uncomfortable due to its strong vibration strength. The designed compound vibration with low vibration strength could effectively prevent bone quality decrease of ovariectomized rats. OBJECTIVE: To verify the effect of compound vibration with low vibration strength on the bone quality of ovariectomized rats. METHODS: A total of 32 4-month-old female SD rats were used in this study. All SD rats were randomly dlvided into 4 groups: normal control group, ovadectomized control group, vibration Ⅰ group (45 Hz-55 Hz, 0.05 g-0.1 g), and vibration Ⅱ group (45 Hz-55 Hz, 0.12 g-0.21 g), with8 rats for each group. The intervention to each vibration group was onea day, 20 minutes/tlmes and 5 days per week. The resting interval was shorted than 2 days, and the whole vibration intervention lasted 13 weeks. Bone mineral density (BMD) before and after vibration, ex vivo bone microstructure parameters and biomechanics parameters ware measured in this study. RESULTS AND CONCLUSION: Lumbar BMD of ovariectomized control group was decreased (P < 0.05), but the BMD in the normal control group and the two vibration groups was increased significantly. Femoral BMD of all groups was increased, but there was no significant difference among the four groups. Bone microstructure parameters of the three ovariectomized groups ware decreased; however, parameters including number, thickness and distance of bone trabecula and bone volume fraction in the vibration Ⅱ group were significantly improved compared to ovadactomized control group. Moreover, bone strength of lumbar vertebra in the two vibration groups was significantly increased (P=0.025, 0.006), but there was no significant difference compared to normal control group. Compound vibration with low strength could effectively prevent BMD decrease of ovadectomized rats, lighten the dagrea of bone microstructure damage, keep bone strength, improve bone quality of ovadectomized rats, and potentially prevent osteoporosis.