国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2012年
3期
231-235
,共5页
何书宇%夏福灿%董海燕%孙伟楠%郝增辉%徐红薇
何書宇%夏福燦%董海燕%孫偉楠%郝增輝%徐紅薇
하서우%하복찬%동해연%손위남%학증휘%서홍미
Irgm1%tMCAO%神经元%自噬
Irgm1%tMCAO%神經元%自噬
Irgm1%tMCAO%신경원%자서
Irgm1%tMCAO%Neurons%Auophagy
目的 探讨免疫相关GTP酶1(Irgm1)在小鼠暂时性大脑中动脉缺血再灌注模型(tMCAO)中的表达及其对tMCAO小鼠大脑神经元内自噬发生的影响.方法 采用western blotting和免疫荧光染色,检测Irgm1和自噬相关基因LC3I/Ⅱ及P62在Irgm1+/+小鼠tMCAO脑组织内的表达水平及细胞定位；进一步比较Irgm1+/+及Irgm1-/-小鼠tMCAO大脑神经元内自噬发生的差异.结果 相比于假手术(sham)组,Irgm1在Irgrm1+/+小鼠tMCAO脑组织中表达明显增高,且主要分布于损伤侧皮层神经元内；同时我们也证实在小鼠tMCAO脑组织中LC3-Ⅱ表达增加(t=-16.448,P＜0.01),面P62表达明显减低(t=3.975,P＜0.05).我们进一步实验发现Irgm1-/-小鼠tMCAO脑组织内LC3-Ⅱ表达量低于Irgm1+/+小鼠tMCAO(t=4.073,P＜0.05),而P62表达量高于Irgm1+/+小鼠tMCAO(t=-3.383,P＜0.05).结论 在小鼠tMCAO模型中,Irgm1参与调节大脑神经元的自噬活动.
目的 探討免疫相關GTP酶1(Irgm1)在小鼠暫時性大腦中動脈缺血再灌註模型(tMCAO)中的錶達及其對tMCAO小鼠大腦神經元內自噬髮生的影響.方法 採用western blotting和免疫熒光染色,檢測Irgm1和自噬相關基因LC3I/Ⅱ及P62在Irgm1+/+小鼠tMCAO腦組織內的錶達水平及細胞定位；進一步比較Irgm1+/+及Irgm1-/-小鼠tMCAO大腦神經元內自噬髮生的差異.結果 相比于假手術(sham)組,Irgm1在Irgrm1+/+小鼠tMCAO腦組織中錶達明顯增高,且主要分佈于損傷側皮層神經元內；同時我們也證實在小鼠tMCAO腦組織中LC3-Ⅱ錶達增加(t=-16.448,P＜0.01),麵P62錶達明顯減低(t=3.975,P＜0.05).我們進一步實驗髮現Irgm1-/-小鼠tMCAO腦組織內LC3-Ⅱ錶達量低于Irgm1+/+小鼠tMCAO(t=4.073,P＜0.05),而P62錶達量高于Irgm1+/+小鼠tMCAO(t=-3.383,P＜0.05).結論 在小鼠tMCAO模型中,Irgm1參與調節大腦神經元的自噬活動.
목적 탐토면역상관GTP매1(Irgm1)재소서잠시성대뇌중동맥결혈재관주모형(tMCAO)중적표체급기대tMCAO소서대뇌신경원내자서발생적영향.방법 채용western blotting화면역형광염색,검측Irgm1화자서상관기인LC3I/Ⅱ급P62재Irgm1+/+소서tMCAO뇌조직내적표체수평급세포정위；진일보비교Irgm1+/+급Irgm1-/-소서tMCAO대뇌신경원내자서발생적차이.결과 상비우가수술(sham)조,Irgm1재Irgrm1+/+소서tMCAO뇌조직중표체명현증고,차주요분포우손상측피층신경원내；동시아문야증실재소서tMCAO뇌조직중LC3-Ⅱ표체증가(t=-16.448,P＜0.01),면P62표체명현감저(t=3.975,P＜0.05).아문진일보실험발현Irgm1-/-소서tMCAO뇌조직내LC3-Ⅱ표체량저우Irgm1+/+소서tMCAO(t=4.073,P＜0.05),이P62표체량고우Irgm1+/+소서tMCAO(t=-3.383,P＜0.05).결론 재소서tMCAO모형중,Irgm1삼여조절대뇌신경원적자서활동.
Objective To investigate the impact of immunity-related GTPase Irgm1 on the regulation of neuronal autophagy in tMCAO mice.Methods Brain tissues were collected from Irgm1 +/+ tMCAO mice.Irgm1 and autophagy-related genes LC3-Ⅰ/ Ⅱ and p62 expression were detected by western blotting and immunofluorescence staining.In addition,we compared the expression of autophagy related genes between the brain tissues of Irgm+/+ and Irgm1+/+ mice after tMCAO.Results Irgm1 was increased in the cortex neurons of Irgm1 +/+ mice tMCAO compared to sham group.The increatted Irgml was accompanied by enhanced neuronal autophagic activity proved by higher expression of LC3-Ⅱ ( t =-16.448,P ＜ 0.01 ) and lower expression of p62 (t=3.975,P＜0.05) in Irgm1 +/++ mice tMCAO.However,we found that in Irgm1-/- mice the exprossion of Lc3- Ⅱ was lower ( P ＜ 0.01 ) while the expression of p62 was higher ( P ＜ 0.05 ) compared with that in Irgm1 +/+ mice.Conclusion The increased expression of Irgm1 in the neurons during tMCAO is important for neuronal autophagy.
