CAJ | 학술논문

目的探讨瑞芬太尼后处理对脑缺血再灌注大鼠海马神经元凋亡的影响.方法健康成年雄性SD大鼠24只,体重250～300 g,随机分为4组(n=6):假手术组(S组)、缺血再灌注组(IR组)、瑞芬太尼0.6μg·kg-1·min-1组(R1组)和瑞芬太尼1.8μg·kg-1·min-1组(R2组).采用夹闭双侧颈总动脉联合低血压法制备大鼠全脑缺血再灌注模型.R1组和R2组分别于再灌注前5 min泵注瑞芬太尼0.6、1.8μg·kg-1·min-1,注射时间5 min.于再灌注第3天采用Morris水迷宫实验及跳台实验测定大鼠认知功能.水迷宫实验结束后,处死大鼠取脑分离海马,采用免疫组化法检测海马CA1区caspase-3蛋白的表达,TUNEL法检测神经元凋亡情况.结果与S组相比,IR组、R1组和R2组大鼠认知功能减退,海马CA1区神经元凋亡数目升高,IR组caspase-3表达上调(P＜0.05);与IR组相比,R1组和R2组大鼠认知功能提高,海马CA1区caspase-3表达水平和凋亡神经元数目降低(P＜0.05).结论瑞芬太尼后处理可通过下调caspase-3表达,抑制海马神经元凋亡,从而改善脑缺血再灌注大鼠的认知功能.
목적 탐토서분태니후처리대뇌결혈재관주대서해마신경원조망적영향.방법 건강성년웅성SD대서24지,체중250～300 g,수궤분위4조(n=6):가수술조(S조)、결혈재관주조(IR조)、서분태니0.6μg·kg-1·min-1조(R1조)화서분태니1.8μg·kg-1·min-1조(R2조).채용협폐쌍측경총동맥연합저혈압법제비대서전뇌결혈재관주모형.R1조화R2조분별우재관주전5 min빙주서분태니0.6、1.8μg·kg-1·min-1,주사시간5 min.우재관주제3천채용Morris수미궁실험급도태실험측정대서인지공능.수미궁실험결속후,처사대서취뇌분리해마,채용면역조화법검측해마CA1구caspase-3단백적표체,TUNEL법검측신경원조망정황.결과 여S조상비,IR조、R1조화R2조대서인지공능감퇴,해마CA1구신경원조망수목승고,IR조caspase-3표체상조(P＜0.05);여IR조상비,R1조화R2조대서인지공능제고,해마CA1구caspase-3표체수평화조망신경원수목강저(P＜0.05).결론 서분태니후처리가통과하조caspase-3표체,억제해마신경원조망,종이개선뇌결혈재관주대서적인지공능.
Objective To investigate the effects of remifentanil postconditioning on apoptosis in hippocampal neruons in a rat model of cerebral ischemia-reperfusion(I/R)and the mechanism involved.Methods Twentyfour male SD rats weighing 250-300 g were randomly divided into 4 groups(n = 6 each): sham operation group (group S);global cerebral I/R group(group I/R);remifentanil 0.6μg·kg- 1·min-1+global cerebral I/R group (group R1)and remifentanil 1.8μg·kg-1·min-1 + global cerebral I/R group(group R2).Global cerebral ischemia was induced by 10 min occlusion of bilateral common carotid combined with hypotension.In group R1 and R2,remifentanil at 0.6 and 1.8μg·kg-1·min-1 were infused for 5 min before ischemia respectively.The cognitive function was tested with Morris water maze and step-down tests from the day 3 to day 8 after reperfusion.When Morris water maze test was finished,rat brains were removed for HE staining and determination of the expression of caspase-3 in hippocampal CA1 region by immuno-histochemistry.Apoptosis in neurons in hippocampal CA1 region was detected by TUNEL assay.Results Compared with group S,the cognitive function was significantly decreased and the number of apopotic neurons in hippocampus CA1 region increased in group I/R,R1 and R2,and the expression of caspase-3 was up-regulated in group I/R(P＜0.05).Compared with group I/R,the cognitive function was significantly increased,the expression of caspase-3 was down-regulated,and the number of apopotic neurons in hippocampus CA1 region was significantly decreased in group R1 and R2(P＜0.05).Conclusion Remifentanil postconditioning can improve the cognitive function through down-regulating caspase-3 expression and inhibiting the apoptosis in hippocampal neruons in a rat model of cerebral I/R.