CAJ | 학술논문

目的探讨PCR和FISH检测技术在原发性子宫颈淋巴瘤与淋巴瘤样病变的诊断与鉴别诊断中的作用.方法收集3例原发性子宫颈弥漫性大B细胞淋巴瘤(DLBCL)与2例子宫颈淋巴瘤样病变,进行PCR-IgH重排及FISH检测.结果 PCR检测显示3例DLBCL和1例淋巴瘤样病变中出现单克隆性IgH基因重排.间期FISH检测显示3例DLBCL均发生了IgH和bcl-6基因断裂,而2例淋巴瘤样病变均未检测到特定的染色体断裂.结论 PCR-IgH重排并非仅见于子宫颈细胞淋巴瘤.间期FISH检测IgH和bcl-6基因的断裂对于子宫颈B细胞淋巴瘤和淋巴瘤样病变的诊断和鉴别诊断有帮助.
목적 탐토PCR화FISH검측기술재원발성자궁경림파류여림파류양병변적진단여감별진단중적작용.방법 수집3례원발성자궁경미만성대B세포림파류(DLBCL)여2례자궁경림파류양병변,진행PCR-IgH중배급FISH검측.결과 PCR검측현시3례DLBCL화1례림파류양병변중출현단극륭성IgH기인중배.간기FISH검측현시3례DLBCL균발생료IgH화bcl-6기인단렬,이2례림파류양병변균미검측도특정적염색체단렬.결론 PCR-IgH중배병비부견우자궁경세포림파류.간기FISH검측IgH화bcl-6기인적단렬대우자궁경B세포림파류화림파류양병변적진단화감별진단유방조.
Objective To investigate the role of polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) in diagnosis and differential diagnosis of primary lymphoma and lymphoma-like lesion of the uterine cervix. Methods Three cases of primary cervical diffuse large B-cell lymphoma (DLBCL) and two cases of cervical lymphoma-like lesion were retrospectively studied by detecting their PCRIgH rearrangement and chromosomal break of IgH, bcl-6, bcl-2 and mye genes with FISH. Results PCRIgH gene rearrangement analysis demonstrated that the monoclonal rearrangement of IgH gene was occurred in 3 cases of cervical lymphoma and 1 case of lymphoma-like lesion, Interphase FISH analysis detected IgH gene break and bcl-6 gene break in all the three cases of cervical lymphoma, while no specific chromosomal break of IgH, and bel-6, bcl-2 and myc genes was detected in two cases of cervical lymphoma-like lesion.Conclusion PCR-IgH gene rearrangement is not specific to cervical B-cell lymphoma. Analysis of IgH gene break and bcl-6 gene break with interphase FISH is helpful to differential diagnosis between B-cell lymphoma and lymphoma-like lesion of the uterine cervix.