中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2011年
7期
594-598
,共5页
叶酸%同型半胱氨酸%骨质疏松%去卵巢%大鼠
葉痠%同型半胱氨痠%骨質疏鬆%去卵巢%大鼠
협산%동형반광안산%골질소송%거란소%대서
Folic acid%Homocysteine%Osteoporosis%Ovariectomized%Rat
目的 探讨叶酸对去卵巢大鼠骨质疏松的保护作用.方法 40只3月龄雌性SD大鼠随机分为5组:假手术组、去卵巢组、乙烯雌酚组(乙烯雌酚0.03 mg·kg-1·d-1)、低剂量叶酸组(叶酸5 mg·kg-1·d-1)、高剂量叶酸组(叶酸20 mg·kg-1·d-1).各组大鼠于术后1周开始灌胃给药,治疗10周,假手术组和去卵巢组给予溶媒灌胃.测定大鼠血浆总同型半胱氨酸(tHcy)浓度,骨匀浆中碱性磷酸酶(ALP)和抗酒石酸酸性磷酸酶(TRACP)的水平;取右股骨和腰5椎体进行骨密度和骨生物力学测定,取腰6椎体和左股骨制备HE切片,观察骨组织的形态学变化.结果 与假手术组比较,去卵巢大鼠血浆tHcy浓度明显升高,腰椎和股骨骨密度显著减低(均P＜0.01),血浆tHcy浓度与腰椎骨密度呈负相关(r=-0.359,P=0.040).叶酸显著降低去卵巢大鼠血浆tHcy浓度(均P＜0.01).大剂量叶酸显著增加去卵巢大鼠骨匀浆ALP水平,降低TRACP水平,增加腰椎和股骨骨密度(均P＜0.01),改善腰椎和股骨的生物力学性能.结论 去卵巢大鼠存在高同型半胱氨酸血症,高同型半胱氨酸参与了去卵巢大鼠骨质疏松的发生.叶酸对去卵巢大鼠骨质疏松具有保护作用,其机制可能与改善同型半胱氨酸的代谢作用有关.
目的 探討葉痠對去卵巢大鼠骨質疏鬆的保護作用.方法 40隻3月齡雌性SD大鼠隨機分為5組:假手術組、去卵巢組、乙烯雌酚組(乙烯雌酚0.03 mg·kg-1·d-1)、低劑量葉痠組(葉痠5 mg·kg-1·d-1)、高劑量葉痠組(葉痠20 mg·kg-1·d-1).各組大鼠于術後1週開始灌胃給藥,治療10週,假手術組和去卵巢組給予溶媒灌胃.測定大鼠血漿總同型半胱氨痠(tHcy)濃度,骨勻漿中堿性燐痠酶(ALP)和抗酒石痠痠性燐痠酶(TRACP)的水平;取右股骨和腰5椎體進行骨密度和骨生物力學測定,取腰6椎體和左股骨製備HE切片,觀察骨組織的形態學變化.結果 與假手術組比較,去卵巢大鼠血漿tHcy濃度明顯升高,腰椎和股骨骨密度顯著減低(均P＜0.01),血漿tHcy濃度與腰椎骨密度呈負相關(r=-0.359,P=0.040).葉痠顯著降低去卵巢大鼠血漿tHcy濃度(均P＜0.01).大劑量葉痠顯著增加去卵巢大鼠骨勻漿ALP水平,降低TRACP水平,增加腰椎和股骨骨密度(均P＜0.01),改善腰椎和股骨的生物力學性能.結論 去卵巢大鼠存在高同型半胱氨痠血癥,高同型半胱氨痠參與瞭去卵巢大鼠骨質疏鬆的髮生.葉痠對去卵巢大鼠骨質疏鬆具有保護作用,其機製可能與改善同型半胱氨痠的代謝作用有關.
목적 탐토협산대거란소대서골질소송적보호작용.방법 40지3월령자성SD대서수궤분위5조:가수술조、거란소조、을희자분조(을희자분0.03 mg·kg-1·d-1)、저제량협산조(협산5 mg·kg-1·d-1)、고제량협산조(협산20 mg·kg-1·d-1).각조대서우술후1주개시관위급약,치료10주,가수술조화거란소조급여용매관위.측정대서혈장총동형반광안산(tHcy)농도,골균장중감성린산매(ALP)화항주석산산성린산매(TRACP)적수평;취우고골화요5추체진행골밀도화골생물역학측정,취요6추체화좌고골제비HE절편,관찰골조직적형태학변화.결과 여가수술조비교,거란소대서혈장tHcy농도명현승고,요추화고골골밀도현저감저(균P＜0.01),혈장tHcy농도여요추골밀도정부상관(r=-0.359,P=0.040).협산현저강저거란소대서혈장tHcy농도(균P＜0.01).대제량협산현저증가거란소대서골균장ALP수평,강저TRACP수평,증가요추화고골골밀도(균P＜0.01),개선요추화고골적생물역학성능.결론 거란소대서존재고동형반광안산혈증,고동형반광안산삼여료거란소대서골질소송적발생.협산대거란소대서골질소송구유보호작용,기궤제가능여개선동형반광안산적대사작용유관.
Objective To investigate the protective effect of folic acid(FA) on osteoporosis in ovariectomized(OVX) rats.Methods Forty three-month-old female SD Rats were divided into 5 groups, sham operation group, OVX group, diethylstilbestrol group(0.03mg·kg-1·d-1),low dose FA Group (5 mg·kg-1·d-1),and high dose FA group (20 mg·kg-1·d-1).Gastric gavage in each group was started from one week after being ovariectomized and lasted 10 weeks. Sham operation group and OVX group were treated with solvent. The rats were sacrificed at the end of 10th week after treatment. The total homocysteine(tHcy) in plasma, alkaline phosphatase(ALP), and tartrate-resistant acid phosphatase(TRACP) activity of bone homogenates were measured. The bone mineral density(BMD) and bone biomechanics were determined using L5 vertebrae and right femur. The bone tissue slices were made with L6 vertebrae and left femur and HE stained, and then the histomorphology was observed. Results Compared with sham operation group, plasma tHcy level was significantly increased(P＜0.01), BMD of lumbar vertebrae and femur was remarkedly decreased in OVX group(all P＜0.01). Plasma tHcy concentration was negatively correlated with lumbar BMD(r=-0.359, P=0.040). Plasma tHcy level in both groups treated with folic acid was significantly reduced(all P＜0.01). The ALP concentration in bone homogenates was higher, the TRACP concentration in bone homogenates was lower, and BMD and bone biomechanics of lumbar vertebrae and femur were increased in high dose FA group than those in OVX group(all P ＜0.01). Conclusions In OVX rats hyperhomocysteinemia existed and was involved in the development of osteoporosis. Folic acid could protect OVX rats from osteoporosis, due probably to improved homocysteine metabolism.
