中国医药
中國醫藥
중국의약
CHINA MEDICINE
2009年
8期
583-585
,共3页
桥本病%甲状腺炎,自身免疫性%甲巯咪唑%干扰素Ⅱ型%白细胞介素10
橋本病%甲狀腺炎,自身免疫性%甲巰咪唑%榦擾素Ⅱ型%白細胞介素10
교본병%갑상선염,자신면역성%갑구미서%간우소Ⅱ형%백세포개소10
Hashimoto disease%Thyroiditis autoimmune%Methimazole%Interferon type Ⅱ%Interleukin-10
目的 研究甲巯咪唑(MMI)对实验性自身免疫性甲状腺炎(EAT)的治疗作用,并通过测定IFN-y和IL-10 mRNA的表达水平继而从Th1/Th2
细胞因子失衡角度初步探讨其免疫调节作用的机制.方法 48只昆明小鼠完全随机分为3组,每组16只.EAT组及治疗组用甲状腺球蛋白免疫诱发EAT动物模型;正常组及EAT组给予蒸馏水作为对照;治疗组给予MMI(2 mg/d).所有小鼠观察28 d后处死,分离血清,检测甲状腺球蛋白自身抗体(TGAb)、甲状腺过氧化物酶自身抗体(TPOAb)水平;分离甲状腺进行病理分析并用逆转录聚合酶链反应的方法检测细胞因子IFN-γ和IL-10 mRNA表达水平.结果 EAT组血清TGAb、TPOAb水平约为正常组的5～6倍,造模成功.治疗组较EAT组血清TGAb、TPOAb水平明显降低(P<0.05).治疗组较EAT组IFN-γ mRNA表达水平明显下降,IL-10 mRNA表达水平明显增高(P<0.01).结论 甲巯咪唑对EAT有一定的治疗作用.甲巯咪唑对EAT的保护机制可能与下调,Th1细胞因子、上调Th2细胞因子有关.
目的 研究甲巰咪唑(MMI)對實驗性自身免疫性甲狀腺炎(EAT)的治療作用,併通過測定IFN-y和IL-10 mRNA的錶達水平繼而從Th1/Th2
細胞因子失衡角度初步探討其免疫調節作用的機製.方法 48隻昆明小鼠完全隨機分為3組,每組16隻.EAT組及治療組用甲狀腺毬蛋白免疫誘髮EAT動物模型;正常組及EAT組給予蒸餾水作為對照;治療組給予MMI(2 mg/d).所有小鼠觀察28 d後處死,分離血清,檢測甲狀腺毬蛋白自身抗體(TGAb)、甲狀腺過氧化物酶自身抗體(TPOAb)水平;分離甲狀腺進行病理分析併用逆轉錄聚閤酶鏈反應的方法檢測細胞因子IFN-γ和IL-10 mRNA錶達水平.結果 EAT組血清TGAb、TPOAb水平約為正常組的5～6倍,造模成功.治療組較EAT組血清TGAb、TPOAb水平明顯降低(P<0.05).治療組較EAT組IFN-γ mRNA錶達水平明顯下降,IL-10 mRNA錶達水平明顯增高(P<0.01).結論 甲巰咪唑對EAT有一定的治療作用.甲巰咪唑對EAT的保護機製可能與下調,Th1細胞因子、上調Th2細胞因子有關.
목적 연구갑구미서(MMI)대실험성자신면역성갑상선염(EAT)적치료작용,병통과측정IFN-y화IL-10 mRNA적표체수평계이종Th1/Th2
세포인자실형각도초보탐토기면역조절작용적궤제.방법 48지곤명소서완전수궤분위3조,매조16지.EAT조급치료조용갑상선구단백면역유발EAT동물모형;정상조급EAT조급여증류수작위대조;치료조급여MMI(2 mg/d).소유소서관찰28 d후처사,분리혈청,검측갑상선구단백자신항체(TGAb)、갑상선과양화물매자신항체(TPOAb)수평;분리갑상선진행병리분석병용역전록취합매련반응적방법검측세포인자IFN-γ화IL-10 mRNA표체수평.결과 EAT조혈청TGAb、TPOAb수평약위정상조적5～6배,조모성공.치료조교EAT조혈청TGAb、TPOAb수평명현강저(P<0.05).치료조교EAT조IFN-γ mRNA표체수평명현하강,IL-10 mRNA표체수평명현증고(P<0.01).결론 갑구미서대EAT유일정적치료작용.갑구미서대EAT적보호궤제가능여하조,Th1세포인자、상조Th2세포인자유관.
Objective To study the effect of methimazole on experimental autoimmune thyroiditis(EAT) and to discuss the immuno-regulatory mechanism of Th1/Th2 = cytokine disequilibrium by expressing the levels of IFN-γ and IL-10 mRNA. Methods Forty-eight KM mice were randomly divided into 3 groups with 16 mice in each group: normal control group, EAT control group and Methimazole group. Normal control group and EAT control group were given distilled water as control groups. Methimazole treatment group was given intragastric once every day. All mice were administered drugs 28 days continually. Radio-immunoassay(RIA) was used to test the levels of serum thyroglobulin antibody(TgAb) and thyroid pemxidase antibody(TPOAb). Ten samples of thyroid gland in every group were collected for histological analysis and other thyroid gland tissue were used to assay the levels of cytokines(IFN-γ and IL-10 mRNA)through the methods of reverse transcription-polymerase chain reaction(RT-PCR). Results The level of TGAb and TPOAb in Normal group were 5 ～ 6 times higher EAT group. Compared with EAT group, levels of TGAb and TPOAb in Methimazole treatment group significantly decreased (P < 0.05). Compared with EAT group, the expression of IFN-γ mRNA in Methimazole treatment group significantly decreased (P < 0.01) and the expression of IL-10 mRNA significantly increased (P < 0.05). Conclusions Methimazole has some effects on EAT. The protective function of methimazole may be related to downregulating Th1 cytokine and up-regulating Th2 cytokine.
