中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
2期
255-257
,共3页
李美华%凌峻%熊秋迎%聂艳良
李美華%凌峻%熊鞦迎%聶豔良
리미화%릉준%웅추영%섭염량
颅内动脉瘤%VE-cadherin%β-catenin
顱內動脈瘤%VE-cadherin%β-catenin
로내동맥류%VE-cadherin%β-catenin
Intracranial aneurysm%VE-cadherin%β-catenin
目的 观察血管内皮钙黏蛋白(VE-cadherin)和β-连环蛋白(β-catenin)在SD大鼠和人颅内动脉瘤壁中的表达,探讨血管内皮细胞连接受损与颅内动脉瘤形成的关系.方法 建立肾性高血压诱导的SD大鼠颅内动脉瘤模型,获取8个SD大鼠颅内动脉瘤.另取15例颅内动脉瘤组织标本,应用免疫组织化学方法检测VE-cadherin在大鼠脑动脉瘤壁中的表达,以及VE-cadherin和β-catenin在人脑动脉瘤壁的表达.按照Martina的方法对血管内皮层免疫反应强度进行评分.结果 正常SD大鼠和人的脑动脉血管壁上内皮层完整,VE-cadherin呈棕褐色线样均匀表达.SD大鼠颅内动脉瘤壁上VE-cadherin表达减少,呈间断性非均匀分布.正常SD大鼠脑动脉血管壁和颅内动脉瘤壁免疫反应强度评分分别为2.40 ±0.55和1.38±0.51,两组间差异有统计学意义(t=3.41,P＜0.01).人颅内动脉瘤壁内皮排列紊乱,VE-cadherin和β-catenin表达也明显减少,分布不均匀或表达缺失.正常脑动脉与颅内动脉瘤VE-cadherin和β-catenin免疫反应强度评分分别为2.60±0.54/1.33±0.49和2.80±0.45/1.33±0.49,VE-cadherin和β-catenin在两者间差异均有统计学意义(t分别为4.88和5.91;P＜0.01).结论 颅内动脉瘤的形成过程中存在内皮细胞连接受损.
目的 觀察血管內皮鈣黏蛋白(VE-cadherin)和β-連環蛋白(β-catenin)在SD大鼠和人顱內動脈瘤壁中的錶達,探討血管內皮細胞連接受損與顱內動脈瘤形成的關繫.方法 建立腎性高血壓誘導的SD大鼠顱內動脈瘤模型,穫取8箇SD大鼠顱內動脈瘤.另取15例顱內動脈瘤組織標本,應用免疫組織化學方法檢測VE-cadherin在大鼠腦動脈瘤壁中的錶達,以及VE-cadherin和β-catenin在人腦動脈瘤壁的錶達.按照Martina的方法對血管內皮層免疫反應彊度進行評分.結果 正常SD大鼠和人的腦動脈血管壁上內皮層完整,VE-cadherin呈棕褐色線樣均勻錶達.SD大鼠顱內動脈瘤壁上VE-cadherin錶達減少,呈間斷性非均勻分佈.正常SD大鼠腦動脈血管壁和顱內動脈瘤壁免疫反應彊度評分分彆為2.40 ±0.55和1.38±0.51,兩組間差異有統計學意義(t=3.41,P＜0.01).人顱內動脈瘤壁內皮排列紊亂,VE-cadherin和β-catenin錶達也明顯減少,分佈不均勻或錶達缺失.正常腦動脈與顱內動脈瘤VE-cadherin和β-catenin免疫反應彊度評分分彆為2.60±0.54/1.33±0.49和2.80±0.45/1.33±0.49,VE-cadherin和β-catenin在兩者間差異均有統計學意義(t分彆為4.88和5.91;P＜0.01).結論 顱內動脈瘤的形成過程中存在內皮細胞連接受損.
목적 관찰혈관내피개점단백(VE-cadherin)화β-련배단백(β-catenin)재SD대서화인로내동맥류벽중적표체,탐토혈관내피세포련접수손여로내동맥류형성적관계.방법 건립신성고혈압유도적SD대서로내동맥류모형,획취8개SD대서로내동맥류.령취15례로내동맥류조직표본,응용면역조직화학방법검측VE-cadherin재대서뇌동맥류벽중적표체,이급VE-cadherin화β-catenin재인뇌동맥류벽적표체.안조Martina적방법대혈관내피층면역반응강도진행평분.결과 정상SD대서화인적뇌동맥혈관벽상내피층완정,VE-cadherin정종갈색선양균균표체.SD대서로내동맥류벽상VE-cadherin표체감소,정간단성비균균분포.정상SD대서뇌동맥혈관벽화로내동맥류벽면역반응강도평분분별위2.40 ±0.55화1.38±0.51,량조간차이유통계학의의(t=3.41,P＜0.01).인로내동맥류벽내피배렬문란,VE-cadherin화β-catenin표체야명현감소,분포불균균혹표체결실.정상뇌동맥여로내동맥류VE-cadherin화β-catenin면역반응강도평분분별위2.60±0.54/1.33±0.49화2.80±0.45/1.33±0.49,VE-cadherin화β-catenin재량자간차이균유통계학의의(t분별위4.88화5.91;P＜0.01).결론 로내동맥류적형성과정중존재내피세포련접수손.
Objective To verify whether vascular endothelial intercellular gap injury takes part in the formation of intracranial aneurysms by assaying the expression of vascular endothelial intercellular gap proteins VE-cadherin and β-catenin in the rat experimental intracranial aneurysms and human intracranial aneurysms. Methods Twenty Sprague-Dawley rats were used for establishing a model of hypertension-induced experimental intracranial aneurysm. Eight aneurysmal changes were found in the junction of the anterior cerebralartery (ACA) and the olfactort artery (OA) of rats, and immunohistochemistry was performed to detect the expression level of VE-cadherin. The expression of VE-cadherin and β-catenin was also assayed by using immunohistochemistry in fifteen human intracranial aneurysms. Results The VE-cadherin expression was reduced obviously in the intracranial aneurysms of rats as compared with the nomal cerebral artery ( 1.38 ±0. 51 vs 2.40 ±0. 55 ,P ＜0. 01 ). In human intracranial aneurysms, the expression of VE-cadherin was lower than that in the human superficial temporal artery ( 1.33 ± 0. 49 vs 2. 60 ± 0. 54, P ＜0. 01 ). And the expression of β-catenin was also reduced obviously as compared with the controls ( 1.33 ±0. 49 vs 2. 80 ± 0. 45 ,P ＜0. 01 ). Conclusion Vascular endothelial intercellular gap injury may involve in the formation of intracranial aneurysms.
