CAJ | 학술논문

目的探讨运动训练对大鼠脑出血后神经功能恢复和脑组织中基质金属蛋白酶-9(MMP-9)表达的影响.方法将64只Sprague-Dawley大鼠随机分为运动组及对照组,采用自体血注入法制作脑出血模型.运动组每天给予平衡、抓握、行走等运动训练,对照组置于普通笼内自由活动.在术后第3、7、14和21天进行神经功能评估,并断头取脑组织制成标本,采用免疫组织化学法和原位杂交技术测定脑组织中MMP-9的水平.结果运动组与对照组神经功能评分在第3天最低,第21天评分最高;2组比较,除第3天外,其余各时间点差异均有统计学意义(P<0.05).运动组第3天MMP-9蛋白及mRNA有少量的表达,第7天达到高峰,之后逐渐下降;对照组MMP-9表达在第3天最高,第7天显著下降至最低值;MMP-9蛋白的表达与其mRNA表达变化一致.2组第3天、第7天MMP-9蛋白和mRNA水平比较,差异有统计学意义(P<0.05);而第14天、第21天比较,差异无统计学意义(P>0.05).结论早期运动训练能抑制MMP-9的表达而起到神经保护作用;中期MMP-9的表达增加,并发挥一定的积极作用,推测可能与组织霞构和血管修复从而促进神经功能的恢复有关.
목적 탐토운동훈련대대서뇌출혈후신경공능회복화뇌조직중기질금속단백매-9(MMP-9)표체적영향.방법 장64지Sprague-Dawley대서수궤분위운동조급대조조,채용자체혈주입법제작뇌출혈모형.운동조매천급여평형、조악、행주등운동훈련,대조조치우보통롱내자유활동.재술후제3、7、14화21천진행신경공능평고,병단두취뇌조직제성표본,채용면역조직화학법화원위잡교기술측정뇌조직중MMP-9적수평.결과 운동조여대조조신경공능평분재제3천최저,제21천평분최고;2조비교,제제3천외,기여각시간점차이균유통계학의의(P<0.05).운동조제3천MMP-9단백급mRNA유소량적표체,제7천체도고봉,지후축점하강;대조조MMP-9표체재제3천최고,제7천현저하강지최저치;MMP-9단백적표체여기mRNA표체변화일치.2조제3천、제7천MMP-9단백화mRNA수평비교,차이유통계학의의(P<0.05);이제14천、제21천비교,차이무통계학의의(P>0.05).결론 조기운동훈련능억제MMP-9적표체이기도신경보호작용;중기MMP-9적표체증가,병발휘일정적적겁작용,추측가능여조직하구화혈관수복종이촉진신경공능적회복유관.
Objective To study the effects of exercises training on the recovery of neurological function and the expression of matrix metalloproteinase-9 (MMP-9) in perihematomal brain tissue after intra cerebral hemorrhage (ICH) in rats. Methods Sixty-four male adult Sprague-Dawley rats were randomly divided into exercises group and control group. ICH model was induced by autobiood injection. The rats in exercises group were given balance, grasping and walking exercises every day. The rats in control group exercised freely in standard cages. Neurological function in both groups was measured at the 3rd, 7th, 14th and 21st d. All the rats were sacrificed and the concentration of MMP-9 was measured with immunohistochemical method and in situhybridization. Results In both groups neurological function scores was lowest at the 3rd d, were highest at the 21st d. There was no significant difference between two groups at the 3rd d, but at the 7th, 14th and 21st d the differences between two groups were significant ( P < 0.05 ). In exercises group, MMP-9 expressed weakly at the 3rd d, and peaked at the 7th d, then declined gradually. In control group, MMP-9 expression peaked at the 3rd d, then decreased slowly. MMP-9 protein expression was in accord with mRNA. MMP-9 expression in exer-cises group was significantly different from control group on the 3rd and 7th d (P < 0.05 ) , but not at the 14th and 21st d ( P > 0.05 ). Conclusions Early exercises can inhibit the expression of MMP-9 that could play a role in protecting neurons. Daily exercises can stimulates the expression of MMP-9 so as to have a positive role in midstage of disease. MMP-9 may be involved in tissue remodelling and vascular repairing, which prompt neu-rofunction recovery.