CAJ | 학술논문

人类卵细胞间的胞质转移最近作为人类辅助生殖生物技术的手段并成为研究热点.许多研究表明线粒体对卵母细胞的受精和胚胎发育有显著影响.但是,卵胞质转移伴随着DNA异质即线粒体的异质性.本文对国内外近年来卵胞质转移的研究进展进行综述,并结合克隆技术对其进一步的发展提出自己的见解-逆克隆技术.
인류란세포간적포질전이최근작위인류보조생식생물기술적수단병성위연구열점.허다연구표명선립체대란모세포적수정화배태발육유현저영향.단시,란포질전이반수착DNA이질즉선립체적이질성.본문대국내외근년래란포질전이적연구진전진행종술,병결합극륭기술대기진일보적발전제출자기적견해-역극륭기술.
Cytoplasmic transfer between human oocytes, which represents a complete cytoplasmic exchange, has been performed recently as a means to improve the outcome of assisted reproduction and becomes a hotspot of researches. Many studies have indicated that mitochondria in the oocytoplasm obviously affect fertilization of the oocytes and early embryo development. However, ooplasmic transfer can lead to mitochondrial DNA heteroplasmy and the prospect of mitochondrial heteroplasmy and its potential problems necessitate further studies. The authors reviews the ooplasmic transfer, the relation between ooplasm and fertilization and embryo development, and the mitochondrial heteroplasmy. The authors also propose a new theory of "reverse cloning technique".