吉林农业大学学报
吉林農業大學學報
길임농업대학학보
JOURNAL OF JILIN AGRICUL TURAL UNIVERSITY
2010年
1期
75-80
,共6页
张维%王君玮%李林%赵永刚%任炜杰%王志亮
張維%王君瑋%李林%趙永剛%任煒傑%王誌亮
장유%왕군위%리림%조영강%임위걸%왕지량
犬瘟热病毒%克隆%遗传分析%同源性比较
犬瘟熱病毒%剋隆%遺傳分析%同源性比較
견온열병독%극륭%유전분석%동원성비교
canine distemper virus%cloning%hereditary analysis%homology comparison
为研究鼬科动物犬瘟热病毒(canine distemper virus,CDV)毒株的变异特性,对分离自不同地区貉、貂的5个CDV毒株和1个CDV国内弱毒疫苗株(vacChina)的H基因进行了核苷酸测序,并与12个参考毒株构建系统发生树.结果表明:5个流行毒株之间H基因核苷酸序列同源性均达到97.5%以上,vacChina与5个流行毒株的H基因核苷酸序列同源性普遍较低(90.5%~91.8%),而与国外疫苗株Convac和Onderstepoort的H基因核苷酸同源性达96.8%和97.2%.流行毒株之间H蛋白氨基酸同源性差别不大(97.1%~100.0%),流行毒株与vacChina疫苗株H蛋白氨基酸同源性普遍较低(89.7%~91.8%),vacChina与Onderstepoort、Convac有着很高的同源性,其H蛋白氨基酸同源性分别为97.1%、95.6%.结果分析显示,CDV的流行和免疫失败现象的发生与国内所用疫苗毒株和野毒株的遗传关系甚远有关.
為研究鼬科動物犬瘟熱病毒(canine distemper virus,CDV)毒株的變異特性,對分離自不同地區貉、貂的5箇CDV毒株和1箇CDV國內弱毒疫苗株(vacChina)的H基因進行瞭覈苷痠測序,併與12箇參攷毒株構建繫統髮生樹.結果錶明:5箇流行毒株之間H基因覈苷痠序列同源性均達到97.5%以上,vacChina與5箇流行毒株的H基因覈苷痠序列同源性普遍較低(90.5%~91.8%),而與國外疫苗株Convac和Onderstepoort的H基因覈苷痠同源性達96.8%和97.2%.流行毒株之間H蛋白氨基痠同源性差彆不大(97.1%~100.0%),流行毒株與vacChina疫苗株H蛋白氨基痠同源性普遍較低(89.7%~91.8%),vacChina與Onderstepoort、Convac有著很高的同源性,其H蛋白氨基痠同源性分彆為97.1%、95.6%.結果分析顯示,CDV的流行和免疫失敗現象的髮生與國內所用疫苗毒株和野毒株的遺傳關繫甚遠有關.
위연구유과동물견온열병독(canine distemper virus,CDV)독주적변이특성,대분리자불동지구맥、초적5개CDV독주화1개CDV국내약독역묘주(vacChina)적H기인진행료핵감산측서,병여12개삼고독주구건계통발생수.결과표명:5개류행독주지간H기인핵감산서렬동원성균체도97.5%이상,vacChina여5개류행독주적H기인핵감산서렬동원성보편교저(90.5%~91.8%),이여국외역묘주Convac화Onderstepoort적H기인핵감산동원성체96.8%화97.2%.류행독주지간H단백안기산동원성차별불대(97.1%~100.0%),류행독주여vacChina역묘주H단백안기산동원성보편교저(89.7%~91.8%),vacChina여Onderstepoort、Convac유착흔고적동원성,기H단백안기산동원성분별위97.1%、95.6%.결과분석현시,CDV적류행화면역실패현상적발생여국내소용역묘독주화야독주적유전관계심원유관.
To characterize the variability of recent field isolates of 5 canine distemper virus (CDV) strains from different hosts and geographical areas, we conducted nucleotide sequence analysis of the H gene encoding the haemagglutinin protein. Pronounced differences between 5 field isolates were revealed in comparison to the attenuated vaccine strains vacChina, Convac and Onderstepoort. Phylogenetic tree was constructed with H gene of 5 CDV isolates, vaccine strain vacChina and 12 CDV strains in GeneBank. Results: The homology of nucleic acid sequences among the 5 isolates was high (97.6%-99.9%).The homology of nucleic acid sequences between vacChina and 5 isolates was low(90.5%-91.8%)while high with foreign vaccines,vacConvac(H:96.8%) and vacOnderstepoort(H:97.2%). The difference of amino acid homology of H protein among 5 isolates was not high(97.1%-100.0%). The homology of H protein between vacChina and 5-isolates was low,with H protein 89.7%-91.8%. VacChina and Onderstepoort, Convac had high homology of amino acid in H protein. Conclusion: The CD prevalence in several provinces of China this summer may be due to the far heredity between vaccine strain and field strains.