CAJ | 학술논문

[目的]探索出玉米基因枪转化的条件。[方法]以玉米自交系7239的幼胚胚性愈伤组织为受体材料，研究了轰击距离、气体压力、真空度和轰击次数对转化率的影响。[结果]转化时以金粉用量100μg，枪、发射点与靶细胞距离9cm、气体压力1350psi、真空度25In·Hg、轰击次数2次的组合最佳。经潮霉素（Hygromycin）筛选，获得了再生植株，PCR分析及Southern杂交结果表明ⅣPR1基因已整合到玉米基因组中，平均转化率为1．76％。[结论]该研究为玉米优良抗病品种的培育奠定了基础。
[목적]탐색출옥미기인창전화적조건。[방법]이옥미자교계7239적유배배성유상조직위수체재료，연구료굉격거리、기체압력、진공도화굉격차수대전화솔적영향。[결과]전화시이금분용량100μg，창、발사점여파세포거리9cm、기체압력1350psi、진공도25In·Hg、굉격차수2차적조합최가。경조매소（Hygromycin）사선，획득료재생식주，PCR분석급Southern잡교결과표명ⅣPR1기인이정합도옥미기인조중，평균전화솔위1．76％。[결론]해연구위옥미우량항병품충적배육전정료기출。
[Objective] This study aimed to explore the conditions of transformation of maize by microprojectile bombardment. [Method] Immature embryo-derived callus of maize inbred line 7239 was used as explants to study the effects of shoot distance, helium pressure, vacuum and bombardment frequency on the transformation efficien- cy in the particle bombardment system of maize. [Result] Considering the transfor- mation efficiency, particle bombardment with 100 μg/P of golden particles, at a shoot distance of 9 cm from the target cells, under helium pressure of 1 350 psi and vac- uum 25 inHg, and bombarding twice could achieve relatively ideal results. After se- lection on media supplemented with different concentration of hygromycin, some re- generated plants were obtained. The results of PCR and Southern blotting analysis demonstrated that the NPR1 gene had been integrated into the genome of trans- genic maize plants, with an average transformation efficiency of 1.76%. [Conclusion] The study laid the foundation for the cultivation and breeding of excellent resistant varieties of maize.