CAJ | 학술논문

目的探讨胞质分裂阻滞微核(CBMN)分析在估算辐射事故受照射者的生物剂量中的应用价值.方法 2008年山西太原辐射事故发生后16 h收集5名受照射者(1、2、3、4和5号)的外周血及I号的骨髓,进行CBMN分析,以微核(MN)频率估算生物剂量.对较严重的受照射者(1号)结合体外"co 1射线大剂量照射实验获得的核分裂指数(NDI)估算剂量.照射后1年内对5名受照射者的微核频率进行动态分析.结果 1号患者的微核频率超过了微核剂量-效应曲线上限所对应的数值,用CBMN法结合ND[分析(CBMN+NDI)对其估算的剂量范围为10～20 Gy.以微核频率对2、3、4和5号的剂量估算分别为3.6、2.9、2.3和2.9 Gy.该结果与染色体畸变分析及物理模拟估算的剂量接近.与临床表现基本相符.1号照射后26 d(移植后18 d)微核频率大幅度下降(Ⅱ=3.295,P<0.05).2～5号照射后各时间点所分析的微核频率逐渐减少,照射后1个月时微核频率与照射后16 h相比除2号外,差异均有统计学意义(Ⅱ=6.874、4.526及7.811,P<0.05).结论 CBMN法快速、准确,与染色体畸变分析结合使用可相互补充和验证.新指标CBMN+NDI对于高剂量范围的估计具有参考价值.
목적 탐토포질분렬조체미핵(CBMN)분석재고산복사사고수조사자적생물제량중적응용개치.방법 2008년산서태원복사사고발생후16 h수집5명수조사자(1、2、3、4화5호)적외주혈급I호적골수,진행CBMN분석,이미핵(MN)빈솔고산생물제량.대교엄중적수조사자(1호)결합체외"co 1사선대제량조사실험획득적핵분렬지수(NDI)고산제량.조사후1년내대5명수조사자적미핵빈솔진행동태분석.결과 1호환자적미핵빈솔초과료미핵제량-효응곡선상한소대응적수치,용CBMN법결합ND[분석(CBMN+NDI)대기고산적제량범위위10～20 Gy.이미핵빈솔대2、3、4화5호적제량고산분별위3.6、2.9、2.3화2.9 Gy.해결과여염색체기변분석급물리모의고산적제량접근.여림상표현기본상부.1호조사후26 d(이식후18 d)미핵빈솔대폭도하강(Ⅱ=3.295,P<0.05).2～5호조사후각시간점소분석적미핵빈솔축점감소,조사후1개월시미핵빈솔여조사후16 h상비제2호외,차이균유통계학의의(Ⅱ=6.874、4.526급7.811,P<0.05).결론 CBMN법쾌속、준학,여염색체기변분석결합사용가상호보충화험증.신지표CBMN+NDI대우고제량범위적고계구유삼고개치.
Objective To investigate the value of cytokinesis-block micronuclei(CBMN)assay in estimation of the biological doses of the victims of radiation accident.Methods Samples of peripheral blood were collected from the 5 victims(Subjects 1-5)at 16 h after the radiation accident of Taiyuan,Shanxi Province.And the peripheral blood samples and bone marrow sample were collected from the victim No.1 at 23 and 24 h after the radiation.Eight days after the accident Subject 1 underwent allogeneic peripheral blood hematopoietic stem cell transplantation.At difierent time points in the period of 1 year after the accident.peripheral blood samples were collected from these 5 victims.CBMN assay was conducted on the peripheral blood lymphocytes on the samples,and the biological doses were estimated based on the micronuclei(MN)frequencies.The nuclear division index(NDI)obtained from in vitro irradiation experiment using high dose of 60Coγ-rays was used to estimate the exposed doses for Subject 1. Dynamic arialysis of the MN frequency for the 5 victims was performed in the period of 1 year after the accident.Results The MN frequency of Subject 1 surpassed the value corresponding to the upper limit of the MN dose.effective curve.The dose range estimated bv the combination of the CBMN and NDI (CBMN+NDl)assay was 10-20 Gy for Subject 1.The doses estimated by MN frequency for Subjects 2,3,4,and 5 were 3.6,2.9,2.3,and 2.9 Gy,respectively.The estimated doses were in accordance with those estimated by physicat method.chromosome aberration analysis.and clinical symptoms.Prominent decrease of the MN frequency was observed at 26 d after the accident(18 d after the transplantation)for Subject 1(u=3.295,P<0.05).Gradual decrease of MN frequency was observed after the accident for Subjects 2,3,4,and 5.The MN frequencies 1 month after the accident of Subjects 3,4,and 5 were all significantly lower than those 16 h later(u=6.874,4.526,and 7.811,P<0.05).Conclusions Quick and accurate.CBMN assay reinforces and verifies the result of chromosome aberration analysis.The new index CBMN+NDI assay is of reference valne for estimating higher dose of irradiation.