中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
5期
549-553
,共5页
靳淼%孙军玲%常昭瑞%李慧莹%刘娜%章青%崔淑娴%张静%王子军%段招军
靳淼%孫軍玲%常昭瑞%李慧瑩%劉娜%章青%崔淑嫻%張靜%王子軍%段招軍
근묘%손군령%상소서%리혜형%류나%장청%최숙한%장정%왕자군%단초군
诺如病毒%胃肠炎%暴发%基因分型
諾如病毒%胃腸炎%暴髮%基因分型
낙여병독%위장염%폭발%기인분형
Norovirus%Gastroenteritis%Outbreak%Genotype
目的 了解中国2006-2007年诺如病毒胃肠炎暴发及其病毒基因型别.方法 收集2006-2007年19起胃肠炎暴发的流行病学资料以及腹泻粪便样本,用RT-PCR方法对201份粪便标本进行诺如病毒、轮状病毒、星状病毒、腺病毒和札如病毒检测,对扩增产物进行序列测定.用Clustal X 1.83和MEGA4.0生物软件对诺如病毒序列进行序列比对和系统进化分析.结果 诺如病毒是引起病毒性胃肠炎暴发的主要病原之一(12/19,63.2%).在12起诺如病毒胃肠炎暴发中,变异株GⅡ-4/2006b是引起暴发的流行优势株(11/12,91.7%),其他型别包括GⅡ-17、GⅡ-6和GⅡ-3.诺如病毒引起的疫情暴发主要集中在冬春季节(12月至4月),发生场所多样,且各年龄组人群均有发病.同时,12起诺如病毒暴发中有2起与其他腹泻病毒混合感染,且在同一起暴发中病原具有病毒多样性和基因型别多样性.结论 诺如病毒是2006-2007年引起胃肠炎暴发疫情的主要病原之一,变异株GⅡ-4/2006b为流行优势株.
目的 瞭解中國2006-2007年諾如病毒胃腸炎暴髮及其病毒基因型彆.方法 收集2006-2007年19起胃腸炎暴髮的流行病學資料以及腹瀉糞便樣本,用RT-PCR方法對201份糞便標本進行諾如病毒、輪狀病毒、星狀病毒、腺病毒和札如病毒檢測,對擴增產物進行序列測定.用Clustal X 1.83和MEGA4.0生物軟件對諾如病毒序列進行序列比對和繫統進化分析.結果 諾如病毒是引起病毒性胃腸炎暴髮的主要病原之一(12/19,63.2%).在12起諾如病毒胃腸炎暴髮中,變異株GⅡ-4/2006b是引起暴髮的流行優勢株(11/12,91.7%),其他型彆包括GⅡ-17、GⅡ-6和GⅡ-3.諾如病毒引起的疫情暴髮主要集中在鼕春季節(12月至4月),髮生場所多樣,且各年齡組人群均有髮病.同時,12起諾如病毒暴髮中有2起與其他腹瀉病毒混閤感染,且在同一起暴髮中病原具有病毒多樣性和基因型彆多樣性.結論 諾如病毒是2006-2007年引起胃腸炎暴髮疫情的主要病原之一,變異株GⅡ-4/2006b為流行優勢株.
목적 료해중국2006-2007년낙여병독위장염폭발급기병독기인형별.방법 수집2006-2007년19기위장염폭발적류행병학자료이급복사분편양본,용RT-PCR방법대201빈분편표본진행낙여병독、륜상병독、성상병독、선병독화찰여병독검측,대확증산물진행서렬측정.용Clustal X 1.83화MEGA4.0생물연건대낙여병독서렬진행서렬비대화계통진화분석.결과 낙여병독시인기병독성위장염폭발적주요병원지일(12/19,63.2%).재12기낙여병독위장염폭발중,변이주GⅡ-4/2006b시인기폭발적류행우세주(11/12,91.7%),기타형별포괄GⅡ-17、GⅡ-6화GⅡ-3.낙여병독인기적역정폭발주요집중재동춘계절(12월지4월),발생장소다양,차각년령조인군균유발병.동시,12기낙여병독폭발중유2기여기타복사병독혼합감염,차재동일기폭발중병원구유병독다양성화기인형별다양성.결론 낙여병독시2006-2007년인기위장염폭발역정적주요병원지일,변이주GⅡ-4/2006b위류행우세주.
Objective To acknowledge the epidemiology of gastroenteritis outbreaks caused by norobiruses and their genotypes. Methods Epidemiologic data and specimens were collected from 19 gastroenteritis outbreaks. 201 specimens were detected for norovirus, rotavirus, astrovirus,adenovirus and sapovirus by RT-PCR methods and PCR products were sequenced. Sequence alignment and phylogenetic analysis were performed by Clustal X 1.83 and MEGA 4.0 programs.Results Noroviruses were one of the most predominant pathogens causing viral gastroenteritis outbreaks ( 12 of 19 outbreaks, accounting for 63.2% ). Variant G Ⅱ -4/2006b was the predominant strain responsible for 11 of the 12 NV-associated outbreaks. Other genotypes would include G Ⅱ -17,G Ⅱ -6 and G Ⅱ -3. The NV-associated gastrocnteritis outbreaks occurred mainly in winter and spring between December 2006 and April 2007. These gastroenteritis outbreaks caused by noroviruses would involve all age groups in various locations. Meantime, 2 out of 12 outbreaks were caused by norovirus or other viruses. In addition, multiple viruses and multiple genotypes of noroviruses were found in the same outbreak. Conclusion Noroviruses were one of the most major pathogens causing gastroenteritis outbreaks while G Ⅱ -4/2006b variant was identified as the predominant strain in China.
