中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2009年
3期
180-183
,共4页
雷泽源%黄跃生%肖荣%张兵钱%张琼
雷澤源%黃躍生%肖榮%張兵錢%張瓊
뢰택원%황약생%초영%장병전%장경
烧伤%依那普利拉%灌流%心脏%血管紧张素(1-7)
燒傷%依那普利拉%灌流%心髒%血管緊張素(1-7)
소상%의나보리랍%관류%심장%혈관긴장소(1-7)
Burns%Enalaprilat%Perfusion%Heart%Angiotensin (1-7)
目的 了解血管紧张素(1-7)[Ang(1-7)]和依那普利托对烧伤血清离体灌注大鼠心脏功能的影响.方法 选取80只SD大鼠制备烧伤血清.另取24只SD大鼠心脏建立Langendofff离体灌注模型.根据灌注液不同,将大鼠心脏分为K-H液组、含体积分数20%烧伤血清的K-H液组(烧伤血清组)、含体积分数20%烧伤血清和2μg/mL依那普利拉的K-H液组(依那普利托组)、含体积分数20%烧伤血清和1 nmoL/mL Ang(1-7)的K-H液组[Ang(1-7)组].各组大鼠心脏连续灌注30min上述溶液后,检测其左心室收缩压(LVSP)、左心室舒张末期压(LVEDP)、左心室压力最大上升/下降速率(±dp/dt max),以及冠状动脉血流量(CF)和冠状动脉流出液中肌酸激酶(CK)、乳酸脱氢酶(LDH)的含量.结果 与K-H液组LVSP[(11.2±1.0)kPa,1 kPa=7.5 mm Hg]、+dp/dt max[(642±53)kPa/s]、-dp/dt max[(380±61)kPa/s]及CF值比较,烧伤血清组、依那普利拉组和Ang(1.7)组LVSP[(5.9±0.8)、(8.0±1.1)、(8.9±1.3)kPa]、+dp/dt max[(275±37)、(454±48)、(479±63)kPa/s]、-dp/dt max[(135±35)、(219±47)、(277±58)kPa/s]及CF值均明显降低(P<0.05或P<0.01),LVEDP和冠状动脉流出液中CK、LDH的含量升高.与烧伤血清组比较,依那普利拉组和Ang(1-7)组CF、LVSP及±dp/dt max均明显升高(P<0.05或P<0.01),LVEDP和冠状动脉流出液中CK、LDH的含量明显降低(P<0.01).结论 Ang(1-7)和依那普利拉均能有效改善烧伤血清离体灌注大鼠左心功能,减轻心肌损伤.
目的 瞭解血管緊張素(1-7)[Ang(1-7)]和依那普利託對燒傷血清離體灌註大鼠心髒功能的影響.方法 選取80隻SD大鼠製備燒傷血清.另取24隻SD大鼠心髒建立Langendofff離體灌註模型.根據灌註液不同,將大鼠心髒分為K-H液組、含體積分數20%燒傷血清的K-H液組(燒傷血清組)、含體積分數20%燒傷血清和2μg/mL依那普利拉的K-H液組(依那普利託組)、含體積分數20%燒傷血清和1 nmoL/mL Ang(1-7)的K-H液組[Ang(1-7)組].各組大鼠心髒連續灌註30min上述溶液後,檢測其左心室收縮壓(LVSP)、左心室舒張末期壓(LVEDP)、左心室壓力最大上升/下降速率(±dp/dt max),以及冠狀動脈血流量(CF)和冠狀動脈流齣液中肌痠激酶(CK)、乳痠脫氫酶(LDH)的含量.結果 與K-H液組LVSP[(11.2±1.0)kPa,1 kPa=7.5 mm Hg]、+dp/dt max[(642±53)kPa/s]、-dp/dt max[(380±61)kPa/s]及CF值比較,燒傷血清組、依那普利拉組和Ang(1.7)組LVSP[(5.9±0.8)、(8.0±1.1)、(8.9±1.3)kPa]、+dp/dt max[(275±37)、(454±48)、(479±63)kPa/s]、-dp/dt max[(135±35)、(219±47)、(277±58)kPa/s]及CF值均明顯降低(P<0.05或P<0.01),LVEDP和冠狀動脈流齣液中CK、LDH的含量升高.與燒傷血清組比較,依那普利拉組和Ang(1-7)組CF、LVSP及±dp/dt max均明顯升高(P<0.05或P<0.01),LVEDP和冠狀動脈流齣液中CK、LDH的含量明顯降低(P<0.01).結論 Ang(1-7)和依那普利拉均能有效改善燒傷血清離體灌註大鼠左心功能,減輕心肌損傷.
목적 료해혈관긴장소(1-7)[Ang(1-7)]화의나보리탁대소상혈청리체관주대서심장공능적영향.방법 선취80지SD대서제비소상혈청.령취24지SD대서심장건립Langendofff리체관주모형.근거관주액불동,장대서심장분위K-H액조、함체적분수20%소상혈청적K-H액조(소상혈청조)、함체적분수20%소상혈청화2μg/mL의나보리랍적K-H액조(의나보리탁조)、함체적분수20%소상혈청화1 nmoL/mL Ang(1-7)적K-H액조[Ang(1-7)조].각조대서심장련속관주30min상술용액후,검측기좌심실수축압(LVSP)、좌심실서장말기압(LVEDP)、좌심실압력최대상승/하강속솔(±dp/dt max),이급관상동맥혈류량(CF)화관상동맥류출액중기산격매(CK)、유산탈경매(LDH)적함량.결과 여K-H액조LVSP[(11.2±1.0)kPa,1 kPa=7.5 mm Hg]、+dp/dt max[(642±53)kPa/s]、-dp/dt max[(380±61)kPa/s]급CF치비교,소상혈청조、의나보리랍조화Ang(1.7)조LVSP[(5.9±0.8)、(8.0±1.1)、(8.9±1.3)kPa]、+dp/dt max[(275±37)、(454±48)、(479±63)kPa/s]、-dp/dt max[(135±35)、(219±47)、(277±58)kPa/s]급CF치균명현강저(P<0.05혹P<0.01),LVEDP화관상동맥류출액중CK、LDH적함량승고.여소상혈청조비교,의나보리랍조화Ang(1-7)조CF、LVSP급±dp/dt max균명현승고(P<0.05혹P<0.01),LVEDP화관상동맥류출액중CK、LDH적함량명현강저(P<0.01).결론 Ang(1-7)화의나보리랍균능유효개선소상혈청리체관주대서좌심공능,감경심기손상.
Objective To investigate effects of angiotcnsin ( 1-7 ) [ Ang( 1-7 ) ] and enalaprilat on function of isolated rat heart perfused by burn serum. Methods Eighty SD rats were used to prepare burn serum. Hearts of another 24 SD rats were isolated to reproduce Langendorff perfusion model. The rat hearts were divided into different groups with different perfusion fluids as K-H buffer group, K-H buffer containing 20% burn serum group ( burn serum group) , K-H buffer containing 20% burn serum and 2 μg/mL enala-prilat group (enalaprilat group), and K-H buffer containing 20% burn serum and 1 nmol/mL Ang(1-7) group [ Ang(1-7) group]. The rat hearts were perfused for 30 mins with each of above-mentioned fluids in different groups. Then left ventrieular systolic pressure (LVSP) , left ventricular end diastolic pressure ( LV-EDP) , ± dp/dt max, coronary flow(CF) , level of creatine kinase (CK) and lactate dehydrogenase (LDH) in respective coronary effluent were determined. Results Compared with LVSP ( 11.2 ± 1.0 kPa, 1 kPa = 7.5 mm Hg), +dp/dt max (642±53 kPa/s), -dp/dt max (380±61 kPa/s) and CF level in K-H buffer group, CF, LVSP(5.9±0.8, 8.0±1.1, 8.9±1.3 kPa, respectively), +dp/dt max(275±37, 454±48, 479±63 kPa/s, respectively), - dp/dt max ( 135±35, 219±47, 277±58 kPa/s, respectively ) of burn serum group, those levels in Ang(1-7) group, and enalaprilat group were decreased obviously(P<0.05 or P <0.01 ), but LVEDP, level of CK and LDH in coronary effluent were increased. Compared with those parameters in burn serum group, CF, LVSP, ±dp/dt max of Ang(1-7) group and enalaprilat group were increased obviously(P<0.05 or P<0.01), and LVEDP, level of CK and LDH in coronary effluent were decreased obviously(P<0.01). Conclusions Ang(1-7) and enalaprilat can effectively improve left ventricular function of isolated rat heart perfused by burn serum and mitigate myocardial injury.
