中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2009年
6期
22-25
,共4页
吕娟%索爱琴%李玮%许予明%张杰文%孙争宇
呂娟%索愛琴%李瑋%許予明%張傑文%孫爭宇
려연%색애금%리위%허여명%장걸문%손쟁우
阿尔茨海默病%β-淀粉样肽%白细胞介素-6%丝裂原活化蛋白激酶活化蛋白激酶2%Westernblot%动物模型
阿爾茨海默病%β-澱粉樣肽%白細胞介素-6%絲裂原活化蛋白激酶活化蛋白激酶2%Westernblot%動物模型
아이자해묵병%β-정분양태%백세포개소-6%사렬원활화단백격매활화단백격매2%Westernblot%동물모형
Alzheimer' s disease%Aβ%IL-6%MK2%Western blot%Animal model
目的 探讨β-淀粉样肽(Aβ)诱导阿尔茨海默病(AD)大鼠模型中海马丝裂原活化蛋白激酶活化蛋白激酶2(MK2)和白细胞介素-6(IL-6)的表达及其与老年性记忆力减退的关系,研究炎性机制及信号转导在AD中的作用.方法 采用立体定向下双侧海马注射Aβ1-42建立AD动物模型,通过Y-迷宫测试、HE染色、免疫组化染色及Western blot等方法,观测大鼠学习记忆能力、海马组织结构的病理改变及MK2和IL-6的表达情况.结果 模型组大鼠Y-迷宫学习记忆能力较时照组明显受损(P＜0.05),HE染色显示,模型组大鼠海马神经元变性、缺失,胶质细胞浸润;免疫组化结果显示模型组大鼠海马CAI区MK2和IL-6免疫阳性细胞表达多见(P＜0.05);Western blot显示模型组海马组织MK2条带增宽表达强度高于对照组(P＜0.05),差异有统计学意义.结论 MK2信号转导通路在AD的炎症反应机制中可能具有重要的作用.
目的 探討β-澱粉樣肽(Aβ)誘導阿爾茨海默病(AD)大鼠模型中海馬絲裂原活化蛋白激酶活化蛋白激酶2(MK2)和白細胞介素-6(IL-6)的錶達及其與老年性記憶力減退的關繫,研究炎性機製及信號轉導在AD中的作用.方法 採用立體定嚮下雙側海馬註射Aβ1-42建立AD動物模型,通過Y-迷宮測試、HE染色、免疫組化染色及Western blot等方法,觀測大鼠學習記憶能力、海馬組織結構的病理改變及MK2和IL-6的錶達情況.結果 模型組大鼠Y-迷宮學習記憶能力較時照組明顯受損(P＜0.05),HE染色顯示,模型組大鼠海馬神經元變性、缺失,膠質細胞浸潤;免疫組化結果顯示模型組大鼠海馬CAI區MK2和IL-6免疫暘性細胞錶達多見(P＜0.05);Western blot顯示模型組海馬組織MK2條帶增寬錶達彊度高于對照組(P＜0.05),差異有統計學意義.結論 MK2信號轉導通路在AD的炎癥反應機製中可能具有重要的作用.
목적 탐토β-정분양태(Aβ)유도아이자해묵병(AD)대서모형중해마사렬원활화단백격매활화단백격매2(MK2)화백세포개소-6(IL-6)적표체급기여노년성기억력감퇴적관계,연구염성궤제급신호전도재AD중적작용.방법 채용입체정향하쌍측해마주사Aβ1-42건립AD동물모형,통과Y-미궁측시、HE염색、면역조화염색급Western blot등방법,관측대서학습기억능력、해마조직결구적병리개변급MK2화IL-6적표체정황.결과 모형조대서Y-미궁학습기억능력교시조조명현수손(P＜0.05),HE염색현시,모형조대서해마신경원변성、결실,효질세포침윤;면역조화결과현시모형조대서해마CAI구MK2화IL-6면역양성세포표체다견(P＜0.05);Western blot현시모형조해마조직MK2조대증관표체강도고우대조조(P＜0.05),차이유통계학의의.결론 MK2신호전도통로재AD적염증반응궤제중가능구유중요적작용.
Objective To study the relationship between the changes in the expression of MK2 and IL-6 in hippocampus and senile hypomnesia in the rat model of Alzheimer' s disease(AD)induced by β-amyloid(Aβ),and to explore the effects of inflammatory mechanism and cellular signal transduction pathway in AD.Methods The AD models were established by the injection of Aβ1-42 into the bilateral hippocampus of rats.Observed the alteration of learning and memory abilities,histopathological change in hippocampus and the expression of MK2 and IL-6 by means of Y shape maze test,HE,immunohistochemistry and Western blot.Results Y-maze test showed the abilities of learning and memory in the model group were apparently decreased,in comparision with the control group(P＜0.05).By HE staining,hippocampal neuronal loss,denaturation and diffuse glial reaction in CA1 region were observed in the model group.The number of MK2 and IL-6 imunoreactive neurons within the hippocampal CA1 region in the model group was obviously increased compared with the control group(P＜0.05).The increasing expression of MK2 and the broadening band of MK2 were demonstrated by Western blot in hippocampus in the model group(P＜0.05).Conclusions We suggest that MK2 signal transduction pathway may play an important role in neuroinflammatory pathology in AD.
