CAJ | 학술논문

以山奈的带芽块茎为试验材料,研究其表面消毒的方法和不同配比培养基对山奈块茎诱导、增殖以及生根的影响.结果表明:外植体表面消毒采用体积分数75%酒精消毒1 min,无菌水漂洗1次,然后用质量分数0.1%升汞浸泡10min,无菌水漂洗2次后,再用0.1%升汞浸泡6min,无菌水漂洗4次效果为最佳,污染率可降至6.67%;适宜的诱导培养基为MS+6-BA3.0 mg/L+NAA0.1 mg/L,芽的萌发率达100%,分化率达146.67%;增殖培养基以MS+6-BA3.0 mg/L为最好,增殖倍数可达6.1倍;适宜的生根培养基为MS+NAA0.1 mg/L.生根率可达100%;试管苗移栽到疏松透气的基质中即可,成活率可达93%.
이산내적대아괴경위시험재료,연구기표면소독적방법화불동배비배양기대산내괴경유도、증식이급생근적영향.결과표명:외식체표면소독채용체적분수75%주정소독1 min,무균수표세1차,연후용질량분수0.1%승홍침포10min,무균수표세2차후,재용0.1%승홍침포6min,무균수표세4차효과위최가,오염솔가강지6.67%;괄의적유도배양기위MS+6-BA3.0 mg/L+NAA0.1 mg/L,아적맹발솔체100%,분화솔체146.67%;증식배양기이MS+6-BA3.0 mg/L위최호,증식배수가체6.1배;괄의적생근배양기위MS+NAA0.1 mg/L.생근솔가체100%;시관묘이재도소송투기적기질중즉가,성활솔가체93%.