CAJ | 학술논문

[目的]研究Na+/H+逆向转运蛋白AtNHX1基因对杨树的转化.[方法]以杨树南林895杨的叶片作为外植体,采用根癌农杆菌介导法,构建cre/lox植物表达载体,并对3株转基因植株进行PCR分子检测. [结果]结果表明,较适合的转化条件为:外植体在分化培养基上经过2 d预培养后于OD600 nm值为0.3～0.4的菌液中浸泡7 min,卡那抗性绿苗率可达43.9%;PCR分子检测表明,目的基因已经整合到杨树基因组中.[结论]该系统可以应用于杨树的基因工程研究.
[목적]연구Na+/H+역향전운단백AtNHX1기인대양수적전화.[방법]이양수남림895양적협편작위외식체,채용근암농간균개도법,구건cre/lox식물표체재체,병대3주전기인식주진행PCR분자검측. [결과]결과표명,교괄합적전화조건위:외식체재분화배양기상경과2 d예배양후우OD600 nm치위0.3～0.4적균액중침포7 min,잡나항성록묘솔가체43.9%;PCR분자검측표명,목적기인이경정합도양수기인조중.[결론]해계통가이응용우양수적기인공정연구.
[Objective] The poplar transferred with Na+/H+ anti-porter AtNHX1 gene was studied.[Method] The leaf of the poplar variety--Nanlin 895 being taken as explants, the expression vector cre/lox was set up with Agrobacterium-mediated method, and 3 transgenic plants were detected at molecular level with PCR.[Results] The results showed that the condition of suitable conversion was as follows that after the explants were pre-cultured in the differentiation medium for 2 days, its soaking time in the bacterium with OD600 nm value of 0.3-0.4 was 7 minutes.And then, the ratio of kanamycin-resistant green plant could be 43.9%.PCR molecular detection showed that the target gene had been integrated into poplar genome.[Conclusion] The system could be applied in gene transformation of poplar