CAJ | 학술논문

为了明确几丁质酶在大豆抗大豆胞囊线虫中的作用,测定了不同抗性大豆品种被大豆胞囊线虫侵染前后以及供试诱导剂诱导被豆胞囊线虫侵染后不同抗性大豆品种根内几丁质酶活性变化.结果表明,正常生长情况下,不同抗性大豆品种根内几丁质酶活性无明显差异.接种大豆胞囊线虫后,无论是抗病品种还是感病品种几丁质酶活性均增高.经过几丁质、壳聚糖和低聚糖诱导处理大豆胞囊线虫侵染的大豆后,均能在一定程度上诱导几丁质酶活性进一步增强,并随着时间的延长,酶活性表现出一定的消长趋势;与其他两个诱导剂处理相比,几丁质诱导处理大豆后几丁质酶活性高峰提前,酶活性增加幅度大.
위료명학궤정질매재대두항대두포낭선충중적작용,측정료불동항성대두품충피대두포낭선충침염전후이급공시유도제유도피두포낭선충침염후불동항성대두품충근내궤정질매활성변화.결과표명,정상생장정황하,불동항성대두품충근내궤정질매활성무명현차이.접충대두포낭선충후,무론시항병품충환시감병품충궤정질매활성균증고.경과궤정질、각취당화저취당유도처리대두포낭선충침염적대두후,균능재일정정도상유도궤정질매활성진일보증강,병수착시간적연장,매활성표현출일정적소장추세;여기타량개유도제처리상비,궤정질유도처리대두후궤정질매활성고봉제전,매활성증가폭도대.
Tbe chitinase activity of different resistance soybean roots were determined before and after being infected by soybean cyst nematode and also in different resistance soybean roots infected by soybean cyst nematode and inoculated with elicitors in order to identify the effects of chitinase to resistant SCN.The results showed that the chitinase activity of different resistance soybean varieties with uninoculation were no significant difference.The chitinase activity increased in both the resistance and susceptible soybean after inoculating soybean cyst nematode. And the chitinase activity enlarged to some extent after inoculating with chitin,chitosan and oligosaccharides.As time went on,the chitinase activity showed a certain degree of growth and decline trends.The peak of chitinaso activity was earlier and the range of increasement was larger than the other two elicitors after the inducing treatment of chitinase.