CAJ | 학술논문

采用HPLC-DAD和LC-MS/MS对各种芦荟样品中蒽醌类成分进行鉴定,在此基础上建立了同时测定各种芦荟样品中芦荟苷与芦荟大黄素含量的方法.采用Nucleodur-siliea色谱柱(250mm*4.6mm,5μm),流动相A为甲醇-醋酸(500:1.70),B为水-醋酸(500:1.70),梯度洗脱,流速1.0 mL/min,DAD扫描波长范围为190～370 nm,紫外检测波长为254和356 nm.质谱离子源为ESI,采用全扫描一级质谱和选择离子全扫描二级质谱两种方式同时测定.结果表明:各种芦荟样品中主要的蒽醌类成分为芦荟苷A、B与芦荟大黄素,未检测到大黄素、大黄酸、大黄素甲醚、大黄酚;芦荟干粉中所含的芦荟苷含量最高.该法准确、可靠、重现性好,可行性高.
채용HPLC-DAD화LC-MS/MS대각충호회양품중은곤류성분진행감정,재차기출상건립료동시측정각충호회양품중호회감여호회대황소함량적방법.채용Nucleodur-siliea색보주(250mm*4.6mm,5μm),류동상A위갑순-작산(500:1.70),B위수-작산(500:1.70),제도세탈,류속1.0 mL/min,DAD소묘파장범위위190～370 nm,자외검측파장위254화356 nm.질보리자원위ESI,채용전소묘일급질보화선택리자전소묘이급질보량충방식동시측정.결과표명:각충호회양품중주요적은곤류성분위호회감A、B여호회대황소,미검측도대황소、대황산、대황소갑미、대황분;호회간분중소함적호회감함량최고.해법준학、가고、중현성호,가행성고.
A HPLC-DAD and LC-MS/MS method was established not only for the assay of anthraquinones but also for the determination of aloin and aloe-emodin in different samples of aloe. The analysis was performed on Nucleodur-silica C_(18)(250 mm *4.6 mm,5 μm)column with a methanol-water (containing 0.4% acetic acid) gradient at ambient temperament. The flow rate was 1.0 mL/min, the wavelength scanned from 190-370 nm and the detection was at 254 nm and 356 nm. The ionic source of LC-MS was ESI. Samples were analyzed in two modes: MS and MS/MS full can modes. Aloin A,B and aloe-emodin were the main anthraquinones in different samples of aloe while rhein,emodin,chrysophanol and physcion were not found; the aloe powder contained the highest amount of aloin. The method could be used to control the quality of aloe product.