CAJ | 학술논문

目的:探讨线粒体融合蛋白2(mitofusin 2,Mfn2)在非小细胞肺癌(non-small-cell lung cancer,NSCLC)组织和非癌肺组织中的表达及与临床病理特征之间的关系.方法:应用原位杂交和免疫组织化学法检测92例NSCLC组织和27例非癌肺组织中Mfn2 mRNA及蛋白的表达.结果: 肺鳞癌组织中Mfn2 mRNA和蛋白的阳性表达率(83.33%和89.58%)均高于肺腺癌(56.82%和65.91%),且2者均高于非癌肺组织的阳性表达率(25.93%和29.63%),3组间比较差异有统计学意义(P<0.001和 P<0.01).高分化和中分化的肺癌组织中Mfn2 mRNA和蛋白的阳性表达率(93.75%和100%,91.67%和91.67%)均高于低分化的肺癌组织(21.43%和42.86%),差异有统计学意义(P<0.001).Mfn2 mRNA和蛋白的表达与患者的性别、年龄、肿瘤大小、TNM分期和淋巴结转移无关(P>0.05).肺癌组织中Mfn2 mRNA和蛋白的表达具有较好的一致性.结论:Mfn2参与了肺癌的发生和发展,Mfn2的表达与肺癌的组织学类型及分化程度有关.
목적:탐토선립체융합단백2(mitofusin 2,Mfn2)재비소세포폐암(non-small-cell lung cancer,NSCLC)조직화비암폐조직중적표체급여림상병리특정지간적관계.방법:응용원위잡교화면역조직화학법검측92례NSCLC조직화27례비암폐조직중Mfn2 mRNA급단백적표체.결과: 폐린암조직중Mfn2 mRNA화단백적양성표체솔(83.33%화89.58%)균고우폐선암(56.82%화65.91%),차2자균고우비암폐조직적양성표체솔(25.93%화29.63%),3조간비교차이유통계학의의(P<0.001화 P<0.01).고분화화중분화적폐암조직중Mfn2 mRNA화단백적양성표체솔(93.75%화100%,91.67%화91.67%)균고우저분화적폐암조직(21.43%화42.86%),차이유통계학의의(P<0.001).Mfn2 mRNA화단백적표체여환자적성별、년령、종류대소、TNM분기화림파결전이무관(P>0.05).폐암조직중Mfn2 mRNA화단백적표체구유교호적일치성.결론:Mfn2삼여료폐암적발생화발전,Mfn2적표체여폐암적조직학류형급분화정도유관.
Objective:To investigate the expressions of Mfn2(mitofusin 2) in non-small-cell lung cancer (NSCLC) tissues and non-cancerous lung tissues,and analyze its relationship with clinicopathological characteristics of lung carcinomas.Methods:The expressions of Mfn2 mRNA and protein in 92 cases of NSCLC tissues and 27 cases of non-cancerous lung tissues were detected by in situ hybridization and immunohistochemical methods. Results:The positive rates of Mfn2 mRNA and protein in pulmonary squamous cell carcinoma were higher than those in adenocarcinoma (83.33% and 89.58% vs 56.82% and 65.91%), respectively. The positive rates of Mfn2 mRNA and protein in NSCLC were higher than those in the non-cancerous lung tissues (25.93% and 29.63%) . The difference was statistically significant among the three groups (P<0.001 and P<0.01). The expressions of Mfn2 mRNA and protein in well-differentiated (93.75% and 100%) and moderately-differentiated NSCLC (91.67% and 91.67%) were higher than those in poor-diffe-rentiated NSCLC (21.43% and 42.86%). The difference was significant (P<0.001). The expressions of Mfn2 mRNA and protein had no correlation with the gender, age, tumor size, TNM stage and lymph node metastasis (P>0.05). The expression of Mfn2 mRNA was consistent with that of Mfn2 protein in NSCLC.Conclusion:Mfn2 was involved in the initiation and progression of lung cancer, and the expression of Mfn2 was related to the histological types of lung cancer and its differentiation degree.