CAJ | 학술논문

[目的]鉴定由猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒Ⅱ型(PCV2)引发的猪疫病并进行病原特性分析.[方法]采集湖南湘潭(编号为HN/XT)发病猪的组织和脏器,进行DNA、RNA提取,然后进行PCR扩增、测序;同时采用细胞分离技术进行毒株的分离、鉴定.CSFV和PCV2的鉴定:间接免疫荧光法操作.PRRSV的鉴定:由于PRRSV可致Marc-145细胞发生病变,故可由细胞病变与否作出初步鉴定.[结果]测序分析结果表明,CSFV、PRRSV和PCV2与目前流行毒序列及GenBank下载序列氨基酸同源性分别为90%、94%和96%左右,可见该次猪病疫情至少是由CSFV、PRRSV和PCV2 3种病毒混合感染引起.[结论]该研究可对当前危害养猪业混合感染疾病的流行病学研究和净化控制提供数据.
[목적]감정유저온병독(CSFV)、저번식여호흡종합정병독(PRRSV)、저원배병독Ⅱ형(PCV2)인발적저역병병진행병원특성분석.[방법]채집호남상담(편호위HN/XT)발병저적조직화장기,진행DNA、RNA제취,연후진행PCR확증、측서;동시채용세포분리기술진행독주적분리、감정.CSFV화PCV2적감정:간접면역형광법조작.PRRSV적감정:유우PRRSV가치Marc-145세포발생병변,고가유세포병변여부작출초보감정.[결과]측서분석결과표명,CSFV、PRRSV화PCV2여목전류행독서렬급GenBank하재서렬안기산동원성분별위90%、94%화96%좌우,가견해차저병역정지소시유CSFV、PRRSV화PCV2 3충병독혼합감염인기.[결론]해연구가대당전위해양저업혼합감염질병적류행병학연구화정화공제제공수거.
[Objective] The aim of this study was to identify swine diseases caused by CSFV, PRRSV and PCV2 and thus to analyze its pathogeny chracteristics. [Method] The tissues and viscera of the diseased swine were collected from Xiangtan of Hunan (Code of HN/XT) to extract DNA and RNA for PCR amplification and sequencing. Meanwhile, the virulent strains were isolated and identified by cell separation technology. [Result] The sequencing analysis results showed that the amino acid homology between CSFV, PRRSV, PCV2 and sequences from current prevalent virulent strains or GenBank was about 90%, 94% and 96% respectively. Accordingly, the swine epidemic situation was caused by the mixed infection of three kinds of viruses (CSFV, PRRSV and PCV2) at least. [Conclusion] The research can provide data for the epidemiologic studies and purification control of the mixed infection diseases harmful for the swine breeding industry at present.