CAJ | 학술논문

目的:从焦化废水中筛选苯酚高效降解菌并进行鉴定.方法:在100～1000 mg/L的苯酚为惟-碳源的无机盐培养基上分离出单菌落,测定各菌株的生长曲线以及对苯酚的降解效牢;利用 16S rDNA序列分析结合菌株的形态特征确定各菌株的分类地位.结果:筛选获得4株苯酚降解菌,均能够以苯酚为惟一碳源,在30℃、pH7.0、摇床转速130 r/min、2%的接种量条件下,24h内能将1 000mg/L的苯酚降解91%以上;4株菌可初步鉴定为芽孢杆菌属(ZL1)、产碱杆菌属(ZL2、ZL4)、沙雷氏菌属(ZL3).其中,从焦化废水中分离出高效降解苯酚的沙雷氏菌未见报道.结论:从焦化废水中获得4株苯酚高效降解细菌,对高浓度含酚废水的生物降解具有潜在的应用前景.
목적:종초화폐수중사선분분고효강해균병진행감정.방법:재100～1000 mg/L적분분위유-탄원적무궤염배양기상분리출단균락,측정각균주적생장곡선이급대분분적강해효뢰;이용 16S rDNA서렬분석결합균주적형태특정학정각균주적분류지위.결과:사선획득4주분분강해균,균능구이분분위유일탄원,재30℃、pH7.0、요상전속130 r/min、2%적접충량조건하,24h내능장1 000mg/L적분분강해91%이상;4주균가초보감정위아포간균속(ZL1)、산감간균속(ZL2、ZL4)、사뢰씨균속(ZL3).기중,종초화폐수중분리출고효강해분분적사뢰씨균미견보도.결론:종초화폐수중획득4주분분고효강해세균,대고농도함분폐수적생물강해구유잠재적응용전경.
Objective:To isolate and identify highly efficient phenol -degrading strain from coking wastewater.Method:The microbes were isolated by the inorganic salt medium containing 100 - 1 000mg/L phenol as a sole carbon source in order to obtain efficient phenol - de-grading strain.The growth curve and phenol - degrading efficiency was determined, also the taxonomic statuses of these strains were deter-mined based on morphological features and 16S rDNA sequences analysis.Result: Four highly effective phenol - degrading bacterial strain named ZL1, ZL2, ZL3, ZL4 were isolated and identified, while strain ZL1 belongs to Bacillus cereus, ZL2 and ZL4 belongs to Alcaligenes faecalis ,ZL3 belongs to Serratia sp.The four bacterial strain could utilize phenol as a sole carbon, tolerate phenol of 1000mg/L, and de-grade phenol of 1000mg/L above 91% in 24h when growing on the inorganic salt medium at temperature 30℃, pH 7.0 and 2% inocula-tion amount.Conclusion: Four highly effective phenol - degrading bacterial strain were obtained and have potential application in the bio-degradation of high concentration organic wastewater with phenol substances.