CAJ | 학술논문

目的检测不同月龄和不同血脂水平载脂蛋白E敲除小鼠体内类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein,StAR)的表达水平.方法实验分别选取雄性和雌性1天龄新生鼠、1月龄、3月龄及5月龄载脂蛋白E敲除小鼠(apoE-/-)及对照C57BL/6J小鼠,每组6只,共16组.利用试剂盒检测不同年龄小鼠的血脂水平.利用实时定量RT-PCR及Western blot方法检测不同年龄和血脂水平下,小鼠肝脏中StAR基因和蛋白的表达.结果相比同年龄同性别的正常对照小鼠,载脂蛋白E敲除小鼠血清中含较高水平的低密度脂蛋白(LDL)和较低水平的高密度脂蛋白(HDL).在对照小鼠中,StAR基因和蛋白表达水平随月龄增加逐渐下降;但是在载脂蛋白E敲除小鼠体内,因为血脂水平的提高,StAR基因和蛋白表达水平呈现先增高后降低的趋势.结论 StAR通过调节脂质代谢,可作为一个有效调节动脉粥样硬化以及其他心血管疾病的调节因子.
목적 검측불동월령화불동혈지수평재지단백E고제소서체내류고순격소합성급성조절단백(steroidogenic acute regulatory protein,StAR)적표체수평.방법 실험분별선취웅성화자성1천령신생서、1월령、3월령급5월령재지단백E고제소서(apoE-/-)급대조C57BL/6J소서,매조6지,공16조.이용시제합검측불동년령소서적혈지수평.이용실시정량RT-PCR급Western blot방법검측불동년령화혈지수평하,소서간장중StAR기인화단백적표체.결과 상비동년령동성별적정상대조소서,재지단백E고제소서혈청중함교고수평적저밀도지단백(LDL)화교저수평적고밀도지단백(HDL).재대조소서중,StAR기인화단백표체수평수월령증가축점하강;단시재재지단백E고제소서체내,인위혈지수평적제고,StAR기인화단백표체수평정현선증고후강저적추세.결론 StAR통과조절지질대사,가작위일개유효조절동맥죽양경화이급기타심혈관질병적조절인자.
Objective To detect steroidogenic acute regulatory protein (StAR) expression in apolipoprotein E-deficient mice at different ages and serum lipid levels. Methods Nighty-six C57BL/6J and apoE-/- mice were enrolled, which were divided into 16 groups with 6 mice per group according to age (1 day, 1, 3, 5 months), sex and genotype (C57BL/6J and apoE-/-). The serum lipid levels in C57BL/6J and apoE-/- mice were detected by commercial kits. StAR mRNA and protein expressions in liver were detected by Real-time PCR and Western blot respectively. Results ApoE-/- mice had higher LDL-cholesterol and lower HDL-cholesterol compared with C57BL/6J mice of the same age and sex. StAR mRNA and protein expressions were decreased following with aging in C57BL/6J mice. However, in apoE-/- mice with higher lipid levels, StAR mRNA and protein expressions were changed with the lipid levels other than ages. StAR mRNA and protein increased in the early stage, and then decreased with the increasement of lipids levels. Conclusions StAR could affect lipids levels and may be an effective regulator for atherosclerosis and other cardiovascular diseases.