CAJ | 학술논문

目的利用肿瘤血清蛋白质组分析方法 (serologic proteome analysis,SERPA)对胰腺癌患者血清和正常人血清进行蛋白质组的分析,寻找胰腺癌特异的肿瘤标记物.方法利用HPLC柱去除血清中的自蛋白,通过双向电泳分离蛋白质,经图像采集与分析,切取胰腺癌患者血清和正常人血清的差异蛋白质点,进行质谱分析鉴定.结果共获得4个差异蛋白质.正常人血清含量高而胰腺癌患者血清含量低的蛋白质为胍裂解环化酶激活蛋白2(guanylate cyclase-activating protein 2).胰腺癌患者血清含量高而正常人血清含量低的蛋白质3个,分别为结合珠蛋白2α(haptoglobin 2 alpha)、转甲状腺素蛋白(transthyretin)及KIAA1018蛋白.结论KIAA1018有望成为胰腺癌筛选和早期诊断的肿瘤标记物.
목적 이용종류혈청단백질조분석방법 (serologic proteome analysis,SERPA)대이선암환자혈청화정상인혈청진행단백질조적분석,심조이선암특이적종류표기물.방법 이용HPLC주거제혈청중적자단백,통과쌍향전영분리단백질,경도상채집여분석,절취이선암환자혈청화정상인혈청적차이단백질점,진행질보분석감정.결과 공획득4개차이단백질.정상인혈청함량고이이선암환자혈청함량저적단백질위고렬해배화매격활단백2(guanylate cyclase-activating protein 2).이선암환자혈청함량고이정상인혈청함량저적단백질3개,분별위결합주단백2α(haptoglobin 2 alpha)、전갑상선소단백(transthyretin)급KIAA1018단백.결론KIAA1018유망성위이선암사선화조기진단적종류표기물.
Objective Serologic proteome analysis method (SERPA) was used to compare the different of the serum proteins between normal and pancreatic cancer patients' serum, and to find a new specific pancreatic cancer marker. Methods HPLC was used to eliminate albumin from the serum, two-dimensional electrophoresis was used to separate the proteins. After imaging collection and analysis, the different proteins between pancreatic cancer and normal subjects were cut for mass spectrometry. Results Four discrepancy proteins were obtained. Guanylate cyclase-activating protein 2 was highly expressed in normal serum but not pancreatic cancer. Hp2-alpha, transthyretin and KIAA1018 protein were highly expressed in cancer patients'serum but not normal people. Conclusions KIAA1018 may become a promising tumor marker for screening and early diagnosis of pancreatic cancer.