CAJ | 학술논문

目的探讨噪声暴露强度和累积噪声暴露量(cumulative noise exposure,CNE)对铜锌超氧化物歧化酶基因(SOD1)rs2070424,rs10432782单核苷酸多态性(single nucleotide polymorphisms,SNPs)与噪声性听力损失(noise induced hearing loss,NIHL)易感性关系的影响.方法利用病例对照研究,通过比较同一噪声暴露强度噪声作业人员的左耳3000 Hz频段听阈位移情况,筛选出听阈位移最大的10％个体作为本研究的易感人群组,共201例,听阈位移最小的10％个体作为耐受人群组,共202例,并进行相关的职业卫生调查和问卷调查.噪声暴露强度的检测根据GBZ/T 189.8-2007《工作场所物理因素测量-噪声》,抽取易感人群和耐受人群空腹外周静脉血5 ml用于常规方法抽提基因组DNA,TaqMan探针法化学荧光等位基因鉴别试验检测SNP.结果在噪声暴露强度85～92 dB(A)组内,rs2070424 AA基因型与GG基因型相比,NIHL的患病风险较低,OR值为0.37(95％CI:0.17～0.80).CNE＞82 dB(A)组内,rs2070424 AA基因型是NIHL的保护因素,与GG基因型相比,OR值为0.25(95％CI0.09～0.70).噪声暴露强度85～92 dB(A)组携带rs10432782 GG基因型患NIHL的风险与TT基因型相比OR值为3.17(95％CI:1.16～6.89).GT基因型与TT基因型相比OR值为2.39( 95％CI:1.16～4.97).CNE 75～82 dB(A)组内,携带GG基因型患NIHL的风险与TT基因型相比,OR值为2.35 (95％CI:0.96～5.72),P=0.06,GG基因型可能是NIHL的危险因素.结论噪声暴露强度和累计噪声暴露量对SOD1rs2070424、rs10432782 SNPs与NIHL.易感性关联存在影响.
목적 탐토조성폭로강도화루적조성폭로량(cumulative noise exposure,CNE)대동자초양화물기화매기인(SOD1)rs2070424,rs10432782단핵감산다태성(single nucleotide polymorphisms,SNPs)여조성성은력손실(noise induced hearing loss,NIHL)역감성관계적영향.방법 이용병례대조연구,통과비교동일조성폭로강도조성작업인원적좌이3000 Hz빈단은역위이정황,사선출은역위이최대적10％개체작위본연구적역감인군조,공201례,은역위이최소적10％개체작위내수인군조,공202례,병진행상관적직업위생조사화문권조사.조성폭로강도적검측근거GBZ/T 189.8-2007《공작장소물리인소측량-조성》,추취역감인군화내수인군공복외주정맥혈5 ml용우상규방법추제기인조DNA,TaqMan탐침법화학형광등위기인감별시험검측SNP.결과 재조성폭로강도85～92 dB(A)조내,rs2070424 AA기인형여GG기인형상비,NIHL적환병풍험교저,OR치위0.37(95％CI:0.17～0.80).CNE＞82 dB(A)조내,rs2070424 AA기인형시NIHL적보호인소,여GG기인형상비,OR치위0.25(95％CI0.09～0.70).조성폭로강도85～92 dB(A)조휴대rs10432782 GG기인형환NIHL적풍험여TT기인형상비OR치위3.17(95％CI:1.16～6.89).GT기인형여TT기인형상비OR치위2.39( 95％CI:1.16～4.97).CNE 75～82 dB(A)조내,휴대GG기인형환NIHL적풍험여TT기인형상비,OR치위2.35 (95％CI:0.96～5.72),P=0.06,GG기인형가능시NIHL적위험인소.결론 조성폭로강도화루계조성폭로량대SOD1rs2070424、rs10432782 SNPs여NIHL.역감성관련존재영향.
Objective To explore the effects of noise exposure level and cumulative noise exposure (CNE) on the relationship between rs2070424 and rs10432782 SNPs in SOD1 and the susceptibility to noiseinduced hearing loss (NIHL).Methods A case-control study was performed for investigating the effects of environmental risk factors on the susceptibility to NIHL in 201sensitive workers and 202 resistant workers.A questionnaire was utilized to investigate the occupational health and to identify the occupational risk factors.The noise exposure levels were detected according to the Chinese standard Measurement of noise in the workplace (GBZ/T 189.8-2007).The peripheral blood samples (5 ml blood for each sample) were from sensitive workers and resistant workers.Genomic DNA was extracted on the basis of the standard procedures of Takara kit.SNPs were detected using standard procedures of TaqMan probe allele identification method.Results In group exposed to 85-92 dB noise (A),the risk of NIHL in the subjects with the AA genotype of rs2070424 was lower than that in the subjects with the GG genotype,OR＝0.37 (95％CI:0.17～0.80).In group exposed to ＞ 82dB CNE (A),the AA genotype of rs2070424 is a protective factor of NIHL,as compared with the GG genotype,OR=0.25 (95％CI:0.09～0.70).In group exposed to 85-92 dB noise (A),the risk of NIHL in the subjects with the GG genotype of rs 10432782 was compared with the risk of NIHL in the subjects with the TT genotype,OR=3.17 (95％CI:1.16～6.89).The GT genotype was compared with TT genotype,OR=2.39(95％ CI:1.16～4.97).In group exposed to 75～82 dB CNE (A),the risk of NIHL in the subjects with the GG genotype was compared with the risk of NIHL in the subjects with the TT genotype,OR=2.35 (95％CI:0.96～5.72),P=0.06.The GG genotype may be a risk factor of NIHJ.Conclusion The noise exposure level and CNE may influence the relationship between rs2070424,rs10432782 SNPs in SOD 1 and noise-induced hearing loss.