中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
8期
926-928
,共3页
纳洛酮%钙通道,L型%肌细胞,心脏
納洛酮%鈣通道,L型%肌細胞,心髒
납락동%개통도,L형%기세포,심장
Naloxone%Calcium channels,L-type%Myocytes,cardiac
目的 评价纳洛酮对大鼠心室肌细胞L型钙电流(ICa-L)的影响.方法 采用急性酶解法分离SD大鼠单个心室肌细胞,以全细胞膜片钳技术测定ICa-L.纳洛酮采用累积给药法,记录不同浓度(20和100μg/ml)纳洛酮作用下心室肌细胞ICa-L和给药前、后半激活电压.结果 20和100 μg/ml的纳洛酮分别使L型钙通道峰电流强度从给药前(341±30) pA降至(270±23) pA和(173±21) pA,与20μg/ml纳洛酮相比,100μg/ml纳洛酮给药后L型钙通道峰电流强度降低(P<0.05).20和100μg/ml纳洛酮给药前、后半激活电压比较差异无统计学意义(P＞0.05).结论 纳洛酮可抑制大鼠心室肌细胞L型钙通道,具有心室肌负性肌力作用.
目的 評價納洛酮對大鼠心室肌細胞L型鈣電流(ICa-L)的影響.方法 採用急性酶解法分離SD大鼠單箇心室肌細胞,以全細胞膜片鉗技術測定ICa-L.納洛酮採用纍積給藥法,記錄不同濃度(20和100μg/ml)納洛酮作用下心室肌細胞ICa-L和給藥前、後半激活電壓.結果 20和100 μg/ml的納洛酮分彆使L型鈣通道峰電流彊度從給藥前(341±30) pA降至(270±23) pA和(173±21) pA,與20μg/ml納洛酮相比,100μg/ml納洛酮給藥後L型鈣通道峰電流彊度降低(P<0.05).20和100μg/ml納洛酮給藥前、後半激活電壓比較差異無統計學意義(P＞0.05).結論 納洛酮可抑製大鼠心室肌細胞L型鈣通道,具有心室肌負性肌力作用.
목적 평개납락동대대서심실기세포L형개전류(ICa-L)적영향.방법 채용급성매해법분리SD대서단개심실기세포,이전세포막편겸기술측정ICa-L.납락동채용루적급약법,기록불동농도(20화100μg/ml)납락동작용하심실기세포ICa-L화급약전、후반격활전압.결과 20화100 μg/ml적납락동분별사L형개통도봉전류강도종급약전(341±30) pA강지(270±23) pA화(173±21) pA,여20μg/ml납락동상비,100μg/ml납락동급약후L형개통도봉전류강도강저(P<0.05).20화100μg/ml납락동급약전、후반격활전압비교차이무통계학의의(P＞0.05).결론 납락동가억제대서심실기세포L형개통도,구유심실기부성기력작용.
Objective To evaluate the effect of naloxone on L-type calcium current (ICa-L) in isolated rat ventricular myocytes.Methods Adult SD rats of both sexes aged 8 weeks weighing 200-250 g were used in this study.Single cardiac ventricular myocytes were enzymatically isolated from SD rats.ICa-L was measured in ventricular myocytes and recorded using whole cell patch-clamp technique.Different concentrations (20 and 100 μg/ml) of naloxone were added to the cardiomyocytes.The effect of naloxone on ICa-L was evaluated.Results The peak current of ICa-L Was inhibited by naloxone in a concentration-dependent manner.Naloxone had no significant effect on steady-state activation curve.Conclusion Naloxone inhibits the L-type calcium channel of ventricular myocytes and exerts negative effect on ventricular muscle function.
