CAJ | 학술논문

目的肿瘤放射治疗诱发骨组织损伤,辐射后成骨细胞Ⅰ型胶原的基因表达分析揭示辐射对早期和晚期的成骨细胞功能的影响.方法在体外诱导骨髓基质细胞生成成骨细胞,对其特性进行确定.用聚合酶链式反应(PCR)方法分析了1～4 Gy照射的早期和晚期成骨细胞的Ⅰ型胶原表达.结果与对照组相比,经1～3 Gy剂量照射后的早期成骨细胞Ⅰ型胶原的表达增强(P＜0.05),但随后即培养10d后的晚期成骨细胞Ⅰ型胶原的表达降低；而在4Gy剂量照射下,晚期成骨细胞Ⅰ型胶原的表达明显高于早期成骨细胞的表达(P＜0.01).结论在1～3 Gy辐射后,早期成骨细胞Ⅰ型胶原的表达增强,成骨能力增强；晚期成骨细胞Ⅰ型胶原的表达降低,成骨能力减弱.而4 Gy辐射后,晚期成骨细胞Ⅰ型胶原的表达增强,有可能是其代偿功能表现.
목적 종류방사치료유발골조직손상,복사후성골세포Ⅰ형효원적기인표체분석게시복사대조기화만기적성골세포공능적영향.방법 재체외유도골수기질세포생성성골세포,대기특성진행학정.용취합매련식반응(PCR)방법분석료1～4 Gy조사적조기화만기성골세포적Ⅰ형효원표체.결과여대조조상비,경1～3 Gy제량조사후적조기성골세포Ⅰ형효원적표체증강(P＜0.05),단수후즉배양10d후적만기성골세포Ⅰ형효원적표체강저；이재4Gy제량조사하,만기성골세포Ⅰ형효원적표체명현고우조기성골세포적표체(P＜0.01).결론 재1～3 Gy복사후,조기성골세포Ⅰ형효원적표체증강,성골능력증강；만기성골세포Ⅰ형효원적표체강저,성골능력감약.이4 Gy복사후,만기성골세포Ⅰ형효원적표체증강,유가능시기대상공능표현.
Objective Cancer radio-therapy may induce bone damage of the patients.collagen type I gene expressions in osteoblast after radiation indicates the influence of radiation on the function of early and late osteoblast.Methods Bone marrow stromal cells were differentiated into osteoblasts in vitro.and the characteristics was indentified.The collagen type I expressions in early and late stage osteoblasts exposed to 1～4Gy radiation were examined by RT-PCR.Results Compared to control group,collagen type I gene expressions increased in early osteoblast after 1～3 Gy radiation (P＜0.05),while the gene expressions in late osteoblast that cultured 10 days decreased.Collagen type I gene expression in late stage ostoblast after 4 Gy irradiation was greatly higher than that in early stage osteoblast (P＜0.01).Conclusion After 1～3 Gy irradiation,the collagen type I expression in early osteoblast was enhanced,indicating the increased ability of bone formation.The exposure to 1～3 Gy decreased collagen type I expression in late osteoblast and weakened the ability of bone formation.The result of high expression of collagen type I in late osteoblast after 4 Gy irradiation may be the manifestation of compensatory function.