CAJ | 학술논문

目的利用高效毛细管电泳法比较2种水解方法提纯的脑蛋白水解物中的多肽组分,同时监测进口脑蛋白水解物注射液的多肽组分分布.方法采用固相萃取法提取脑组织中的多肽成分,以PACE/A5500型高效毛细管电泳仪对进口脑蛋白水解物注射液和本实验室提取的脑组织匀浆物进行比较.结果进口脑蛋白水解物注射液中多肽成分大部分集中在＞14400区域,本实验室提纯的脑蛋白水解物成分中多肽多集中在相对分子质量6000 ～ 14 400区域,且纯度及含量均较高.结论高效毛细管电泳法可有效地对脑蛋白水解物注射液中多肽组分进行分析和比较,2种提纯方法对脑蛋白的裂解能力相差不大.
목적 이용고효모세관전영법비교2충수해방법제순적뇌단백수해물중적다태조분,동시감측진구뇌단백수해물주사액적다태조분분포.방법 채용고상췌취법제취뇌조직중적다태성분,이PACE/A5500형고효모세관전영의대진구뇌단백수해물주사액화본실험실제취적뇌조직균장물진행비교.결과 진구뇌단백수해물주사액중다태성분대부분집중재＞14400구역,본실험실제순적뇌단백수해물성분중다태다집중재상대분자질량6000 ～ 14 400구역,차순도급함량균교고.결론 고효모세관전영법가유효지대뇌단백수해물주사액중다태조분진행분석화비교,2충제순방법대뇌단백적렬해능력상차불대.
Objective To extract the protein hydrolysis ingredients of brain tissue were extracted and to analyze the components of polypeptide in cerebrolysin by using the method of high-performance capillary electrophoresis (HPCE).Methods Brain homogenate were extracted by solid phase extraction method.Cerebrolysin and brain tissue hydrolyzated extracted in lab were detected by HPCE (PACE/A5500,Beckman).Result The result of HPCE showed that the cerebrolysin injections molecular weight of most polypeptides were more than 14 400.The purity and content of cerebrolysin was better and the molecular weights were mainly between 6000 and 14 400.Conclusion The method of HPCE can be effectively used in analyzing components of polypeptides of brain tissue hydrolyzated.There is no difference of protein hydrolysis ingredients of brain tissue extracted by 2 kinds of extraction methods.