中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
7期
655-659
,共5页
郑瑞娟%秦莲花%王伟炳%金瑞良%冯永红%崔振玲%杨华%刘忠华%王洁%陆俊梅%胡忠义
鄭瑞娟%秦蓮花%王偉炳%金瑞良%馮永紅%崔振玲%楊華%劉忠華%王潔%陸俊梅%鬍忠義
정서연%진연화%왕위병%금서량%풍영홍%최진령%양화%류충화%왕길%륙준매%호충의
DC-SIGN基因%结核%多态性%易感性
DC-SIGN基因%結覈%多態性%易感性
DC-SIGN기인%결핵%다태성%역감성
DC-SIGN gene%Tuberculosis%Polymorphism%Disease susceptibility
目的 研究DC-SIGN基因启动子区-871A/G和-336A/G位点多态性和结核病易感性之间的关系.方法 采用病例-对照研究方法,应用焦磷酸测序技术对237例结核病患者和244例健康对照者DC-SIGN基因-871和-336位点进行基因分型,x2检验分析这两个位点多态性与结核病发生的相关性以及与结核病临床特征之间的相关性.结果 DC-SIGN基因-871位点携带有G等位基因的A/G+G/G基因型和不携带有G等位基因的A/A基因型在结核组的频率分别为37.6%、62.4%,在对照组为43.4%、56.6%,二者差别无统计学意义;-336位点携带有G等位基因的A/G+G/G基因型和不携带有G等位基因的A/A基因型在结核组的频率分别为12.2%、87.8%,在对照组为14.3%、85.7%,二者差别亦无统计学意义.中国汉族人群DC-SIGN基因-871和-336位点的G等位基因频率分别为23.2%和7.3%.-336位点A/A和A/G基因型频率在结核发热病人中的差异具有显著性(P=0.037,OR=0.191,95%CI:0.040～0.907).结论 中国汉族人群DC-SIGN基因-871和-336位点多态性与肺结核遗传易感性不相关,-336 G等位基因对于结核病人的发热可能具有保护作用.
目的 研究DC-SIGN基因啟動子區-871A/G和-336A/G位點多態性和結覈病易感性之間的關繫.方法 採用病例-對照研究方法,應用焦燐痠測序技術對237例結覈病患者和244例健康對照者DC-SIGN基因-871和-336位點進行基因分型,x2檢驗分析這兩箇位點多態性與結覈病髮生的相關性以及與結覈病臨床特徵之間的相關性.結果 DC-SIGN基因-871位點攜帶有G等位基因的A/G+G/G基因型和不攜帶有G等位基因的A/A基因型在結覈組的頻率分彆為37.6%、62.4%,在對照組為43.4%、56.6%,二者差彆無統計學意義;-336位點攜帶有G等位基因的A/G+G/G基因型和不攜帶有G等位基因的A/A基因型在結覈組的頻率分彆為12.2%、87.8%,在對照組為14.3%、85.7%,二者差彆亦無統計學意義.中國漢族人群DC-SIGN基因-871和-336位點的G等位基因頻率分彆為23.2%和7.3%.-336位點A/A和A/G基因型頻率在結覈髮熱病人中的差異具有顯著性(P=0.037,OR=0.191,95%CI:0.040～0.907).結論 中國漢族人群DC-SIGN基因-871和-336位點多態性與肺結覈遺傳易感性不相關,-336 G等位基因對于結覈病人的髮熱可能具有保護作用.
목적 연구DC-SIGN기인계동자구-871A/G화-336A/G위점다태성화결핵병역감성지간적관계.방법 채용병례-대조연구방법,응용초린산측서기술대237례결핵병환자화244례건강대조자DC-SIGN기인-871화-336위점진행기인분형,x2검험분석저량개위점다태성여결핵병발생적상관성이급여결핵병림상특정지간적상관성.결과 DC-SIGN기인-871위점휴대유G등위기인적A/G+G/G기인형화불휴대유G등위기인적A/A기인형재결핵조적빈솔분별위37.6%、62.4%,재대조조위43.4%、56.6%,이자차별무통계학의의;-336위점휴대유G등위기인적A/G+G/G기인형화불휴대유G등위기인적A/A기인형재결핵조적빈솔분별위12.2%、87.8%,재대조조위14.3%、85.7%,이자차별역무통계학의의.중국한족인군DC-SIGN기인-871화-336위점적G등위기인빈솔분별위23.2%화7.3%.-336위점A/A화A/G기인형빈솔재결핵발열병인중적차이구유현저성(P=0.037,OR=0.191,95%CI:0.040～0.907).결론 중국한족인군DC-SIGN기인-871화-336위점다태성여폐결핵유전역감성불상관,-336 G등위기인대우결핵병인적발열가능구유보호작용.
Objective To study the relationship of two variants( -871A/G and -336A/G) polymorphisms of the DC-SIGN gene with the susceptibility to pulmonary tuberculosis in Chinese population.Methods Two hundred and thirty-seven tuberculosis cases and 244 controls were genotyped by pyrosequencing in this case-control study. The analysis of the relationship of the -871A/G and -336A/G polymorphisms with their susceptibility of pulmonary tuberculosis(PTB) and the relationship of the two variants with their clinical correlation of tuberculosis was performed by chi-square test. Results The genotypic frequencies of A/G + G/G and A/A of - 871, 37.6%, 62.4% respectively in cases, and 43.4%, 56. 6%respectively in controls, had no significant difference in statistics. And the genotypic frequencies of A/G + G/G and A/A of -336, 12. 2% ,87.8% respectively in cases, and 14.3% ,85.7% respectively in controls, had also no statistical difference between two groups. Interestingly, a significant association is disclosed between the promoter variant - 336G allele and fever in patients ( P = 0. 037, OR = 0. 191, 95 % CI:0. 040-0. 907 ). Conclusion The single nucleotide polymorphism of -871A/G and -336A/G in DCSIGN gene promoter might not be associated with the susceptibility to tuberculosis in Chinese. Tuberculosis patients with -336G allele are significantly protected fever.
