中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
2期
112-116
,共5页
张爱青%李学良%蒋春樱%林琳%施瑞华
張愛青%李學良%蔣春櫻%林琳%施瑞華
장애청%리학량%장춘앵%림림%시서화
Nesfatin-1蛋白%Nucleobindin2蛋白%胰腺%胃%十二指肠%Brunner腺%免疫组织化学
Nesfatin-1蛋白%Nucleobindin2蛋白%胰腺%胃%十二指腸%Brunner腺%免疫組織化學
Nesfatin-1단백%Nucleobindin2단백%이선%위%십이지장%Brunner선%면역조직화학
Nesfatin-1 protein%Nucleobindin2 protein%Pancreas%Stomach%Duodenum%Brunner glands%Immunohistochemistry
目的 探讨一种新的摄食调节肽nesfatin-1/NUCB2在人、SD大鼠及ICR小鼠消化系统的分布,为进一步研究其在消化系统的功能奠定形态学基础.方法 取20例南京医科大学附属第一医院因消化系统疾病而行手术者的手术切除标本共27个,SD大鼠及ICR小鼠的胰腺、胃、十二指肠、食管、肝、小肠、结肠组织.其中恶性肿瘤患者标本为距肿瘤5 cm以上的癌旁组织,良性肿瘤患者的标本为病变边缘组织.免疫组化检测nesfatin-1/NUCB2的分布状况,Western印迹检测NUCB2蛋白的含量.结果 免疫组化显示nesfatin-1/NUCB2免疫阳性细胞在人、SD大鼠和ICR小鼠消化系统中的表达部位大致相似,主要分布于胰腺胰岛的中央;胃黏膜腺下1/3至中1/2的内分泌细胞及十二指肠黏膜下层的布氏腺中.在人、SD大鼠和ICR小鼠组织中均检测到了NUCB2蛋白的表达,但胰腺(分别为0.84±0.03、0.84±0.05和0.84±0.04)、胃(分别为0.86±0.06、0.81±0.02和0.78±0.02)及十二指肠(分别为0.79±0.09、0.79±0.04和0.78±0.05)处的NUCB2蛋白含量显著高于食管(分别为0.43±0.04、0.44±0.02和0.47±0.06)、肝(分别为0.42±0.01、0.44±0.04和0.43±0.01)、小肠(分别为0.32±0.04、0.32±0.04和0.34±0.04)及结肠(分别为0.29±0.01、0.32±0.03和0.28±0.03)中的蛋白含量(P值均=0.000).结论 nesfatin-1/NUCB2这种新的摄食调节肽广泛存在于人、SD大鼠及ICR小鼠的胰岛、胃黏膜腺内分泌细胞及十二指肠布氏腺中,提示其在外周摄食调节、碳水化合物代谢及胃肠功能的调节方面存在一定的功能.
目的 探討一種新的攝食調節肽nesfatin-1/NUCB2在人、SD大鼠及ICR小鼠消化繫統的分佈,為進一步研究其在消化繫統的功能奠定形態學基礎.方法 取20例南京醫科大學附屬第一醫院因消化繫統疾病而行手術者的手術切除標本共27箇,SD大鼠及ICR小鼠的胰腺、胃、十二指腸、食管、肝、小腸、結腸組織.其中噁性腫瘤患者標本為距腫瘤5 cm以上的癌徬組織,良性腫瘤患者的標本為病變邊緣組織.免疫組化檢測nesfatin-1/NUCB2的分佈狀況,Western印跡檢測NUCB2蛋白的含量.結果 免疫組化顯示nesfatin-1/NUCB2免疫暘性細胞在人、SD大鼠和ICR小鼠消化繫統中的錶達部位大緻相似,主要分佈于胰腺胰島的中央;胃黏膜腺下1/3至中1/2的內分泌細胞及十二指腸黏膜下層的佈氏腺中.在人、SD大鼠和ICR小鼠組織中均檢測到瞭NUCB2蛋白的錶達,但胰腺(分彆為0.84±0.03、0.84±0.05和0.84±0.04)、胃(分彆為0.86±0.06、0.81±0.02和0.78±0.02)及十二指腸(分彆為0.79±0.09、0.79±0.04和0.78±0.05)處的NUCB2蛋白含量顯著高于食管(分彆為0.43±0.04、0.44±0.02和0.47±0.06)、肝(分彆為0.42±0.01、0.44±0.04和0.43±0.01)、小腸(分彆為0.32±0.04、0.32±0.04和0.34±0.04)及結腸(分彆為0.29±0.01、0.32±0.03和0.28±0.03)中的蛋白含量(P值均=0.000).結論 nesfatin-1/NUCB2這種新的攝食調節肽廣汎存在于人、SD大鼠及ICR小鼠的胰島、胃黏膜腺內分泌細胞及十二指腸佈氏腺中,提示其在外週攝食調節、碳水化閤物代謝及胃腸功能的調節方麵存在一定的功能.
목적 탐토일충신적섭식조절태nesfatin-1/NUCB2재인、SD대서급ICR소서소화계통적분포,위진일보연구기재소화계통적공능전정형태학기출.방법 취20례남경의과대학부속제일의원인소화계통질병이행수술자적수술절제표본공27개,SD대서급ICR소서적이선、위、십이지장、식관、간、소장、결장조직.기중악성종류환자표본위거종류5 cm이상적암방조직,량성종류환자적표본위병변변연조직.면역조화검측nesfatin-1/NUCB2적분포상황,Western인적검측NUCB2단백적함량.결과 면역조화현시nesfatin-1/NUCB2면역양성세포재인、SD대서화ICR소서소화계통중적표체부위대치상사,주요분포우이선이도적중앙;위점막선하1/3지중1/2적내분비세포급십이지장점막하층적포씨선중.재인、SD대서화ICR소서조직중균검측도료NUCB2단백적표체,단이선(분별위0.84±0.03、0.84±0.05화0.84±0.04)、위(분별위0.86±0.06、0.81±0.02화0.78±0.02)급십이지장(분별위0.79±0.09、0.79±0.04화0.78±0.05)처적NUCB2단백함량현저고우식관(분별위0.43±0.04、0.44±0.02화0.47±0.06)、간(분별위0.42±0.01、0.44±0.04화0.43±0.01)、소장(분별위0.32±0.04、0.32±0.04화0.34±0.04)급결장(분별위0.29±0.01、0.32±0.03화0.28±0.03)중적단백함량(P치균=0.000).결론 nesfatin-1/NUCB2저충신적섭식조절태엄범존재우인、SD대서급ICR소서적이도、위점막선내분비세포급십이지장포씨선중,제시기재외주섭식조절、탄수화합물대사급위장공능적조절방면존재일정적공능.
Objective To investigate the regional distribution and morphological features of nesfatin-1/NUCB2 in digestive system of the humans, Sprague-Dawley (SD) rats and the institute of cancer research (ICR) mice, so as to lay the foundation for further study of its functions in the digestive system. Methods The specimens were obtained from SD rats and ICR mice as well as 20 patients with digestive disease, who were admitted to the First hospital affiliated to Nanjing Medical University and receired surgercal treatment. The specimens from patients with malignant tumors were obtained 5 cm apart from cancerous tissues and from patients with benign tumors were obtained near the focus. The resected tissues included pancreas, stomach, duodenum, esophagus, liver, small intestine or colon. The distribution of nesfatin-1/NUCB2 was examined with immunohistochemical (IHC) staining and its protein level in each organ was measured using Western blotting. Results The immuinohistocemical study revealed the similar distribution pattern of nesfatin-1/NUCB2 in the digestive system of the patients, SD rats and ICR mice. Nesfatin-1/NUCB2 was found to localize in the center of the pancreatic islets, the lower 1/3 to 1/2 of the gastric mucosal glands, as well as the submucosa of the duodenum. Western blotting examination showed the expression of NUCB2 in all tissues from patients, SD rats and ICR mice, whereas the protein level of the nesfatin-1/NUCB2 was higher in pancreas (0.84±0.03, 0. 84±0.05 and 0. 84±0.04, respectively), stomach (0.86±0.06,0.81±0.02 and 0. 78±0.02, respectively) or duodenum (0.79±0.09,0. 79±0.04 and 0.78±0.05)than that in esophagus (0.43±0.04,0.44 ± 0.02 and 0.47 ± 0.06, respectively), liver (0.42±0.01,0.44±0.04 and 0.43 ± 0.01, objectively), small intestine (0.32±0.04,0. 32 ± 0. 04 and 0.34 ±0.04, respectively) or colon (0. 29±0.01,0.32±0.03 and 0. 28±0.03, respectively)(all P values=0. 000). Conclusion Nesfatin-1/NUCB2 is widely expressed in the pancreatic islets, gastric mucosal glands and duodenum of the patients, SD rats and ICR mice, which indicates that nesfatin-1/NUCB2 may be involved in the regulation of food intake, carbohydrate metabolism and gastrointestinal motility.
