CAJ | 학술논문

目的以单纯胰岛素为对照,采用傅立叶红外转换光谱(Fourier Transform Infrared FTIR)研究包裹在脂质体内部胰岛素二级结构的变化.方法分别对单纯胰岛素、胰岛素与空白脂质体混合物(样品Ⅰ)及包裹胰岛素的脂质体(样品Ⅱ)样品进行FTIR测定.结果与单纯胰岛素相比,样品Ⅰ和Ⅱ与胰岛素的FTR谱图形状基本一致,仅其中的α-螺旋略有下降(由36%分别到32%～31%),β-折叠略有增加(由48%分别到53%～51%).样品Ⅰ同样品Ⅱ相比,胰岛素的二级结构无明显差别(α-螺旋分别为32%～31%,β-折叠分别为53%～51%).说明包裹在脂质体内的胰岛素未与脂质体的膜发生插膜作用,所产生与单纯胰岛素二级结构之间微小差别的原因是由于部分胰岛素铺展在脂质体膜表面所致的.结论 FTIR测定被载体包裹的蛋白多肽药物的二级结构,具有快速直接和非破坏性的特点.经FTIR测定,包裹在脂质体内的胰岛素与单纯胰岛素的二级结构相比无明显的变化,仍保持原胰岛素的二级结构.
목적이단순이도소위대조,채용부립협홍외전환광보(Fourier Transform Infrared FTIR)연구포과재지질체내부이도소이급결구적변화.방법분별대단순이도소、이도소여공백지질체혼합물(양품Ⅰ)급포과이도소적지질체(양품Ⅱ)양품진행FTIR측정.결과여단순이도소상비,양품Ⅰ화Ⅱ여이도소적FTR보도형상기본일치,부기중적α-라선략유하강(유36%분별도32%～31%),β-절첩략유증가(유48%분별도53%～51%).양품Ⅰ동양품Ⅱ상비,이도소적이급결구무명현차별(α-라선분별위32%～31%,β-절첩분별위53%～51%).설명포과재지질체내적이도소미여지질체적막발생삽막작용,소산생여단순이도소이급결구지간미소차별적원인시유우부분이도소포전재지질체막표면소치적.결론 FTIR측정피재체포과적단백다태약물적이급결구,구유쾌속직접화비파배성적특점.경FTIR측정,포과재지질체내적이도소여단순이도소적이급결구상비무명현적변화,잉보지원이도소적이급결구.
Aim To determine the secondary structure of insulin encapsulated within liposome. Methods The secondary structure of native insulin, mixture of insulin with liposome(sample Ⅰ)and insulin encapsulated within liposome(sample Ⅱ)were determined by FTIR (Fourier Transform Infrared) spectroscopy. Results The secondary structure of insulin encapsulatedwithin liposome(Ⅱ) are similar with the secondary structure of native insulin. The difference existed in the amount of α-helices(from 36% of insulin to 31% of sample Ⅱ) and β-sheet(from 48% of insulin to 51% of sample Ⅱ). The content of α-helicesand β-sheet of insulin in sample Ⅰ was found to be very close to that of sample Ⅱ. The results revealed that the insulin encapsulated within liposome possibly spread on the surface of liposome, without inserting into the liposome membrane. ConclusionThe secondary structure of insulin encapsulated within liposome is similar with the native insulin.