CAJ | 학술논문

肝细胞生成素(HPO)具有复杂的生理功能,在睾丸中的高表达提示其在生殖活动中的重要性,而不仅局限于肝再生.构建了酵母表达载体pGBKT7-HPO,采用酵母双杂交系统,以HPO为诱饵蛋白,从人睾丸cDNA文库中寻找能够与HPO相互作用的蛋白质.经过筛选、验证阳性克隆,并进行PCR、测序和序列比对,得到4种相互作用蛋白质:NADH脱氢酶1、钠/钾ATP酶β3亚基、磷脂酶C δ1以及附睾分泌蛋白.提示HPO可能参与了细胞的蛋白质合成,能量代谢等.通过对候选蛋白的研究,为探讨HPO对睾丸组织细胞功能的调节机制提供了重要的线索.
간세포생성소(HPO)구유복잡적생리공능,재고환중적고표체제시기재생식활동중적중요성,이불부국한우간재생.구건료효모표체재체pGBKT7-HPO,채용효모쌍잡교계통,이HPO위유이단백,종인고환cDNA문고중심조능구여HPO상호작용적단백질.경과사선、험증양성극륭,병진행PCR、측서화서렬비대,득도4충상호작용단백질:NADH탈경매1、납/갑ATP매β3아기、린지매C δ1이급부고분비단백.제시HPO가능삼여료세포적단백질합성,능량대사등.통과대후선단백적연구,위탐토HPO대고환조직세포공능적조절궤제제공료중요적선색.
Hepatopoietin (HPO) has diverse functions in the regulation of cell growth and differentiation. Its high level expression in testis suggests that HPO may have important role for testicular physiological functions. Using HPO as "bait", a yeast two-hybrid library screen of human testis was performed. By screening and selecting the positive colonies, retesting interactions in yeast, amplifying the AD/library inserts, sequencing and sequence comparing, four HPO-interacting proteins were identified: NADH dehydrogenase 1, Na+/K+ ATPase beta-3 subunit (ATP1β3), phospholipase C delta 1 and epididymal secretory protein. The significance of identification of these proteins may provide mechanistic insight into the biologic role of HPO in testis.