CAJ | 학술논문

采用框栽的培养方法,通过施用不同形态氮肥,探讨不同形态氮对大豆根瘤生长、含氮量、氮积累量以及固氮酶活性的影响.结果表明:不同形态的氮源,对根瘤干重、数量有促进作用,同时增加了根瘤中的含氮量和氮积累量.根瘤干重及数量的变化均为铵态氮(N2)＞蛋白氮(N4)＞硝态氮(N1)＞氨基酸态氮(N3)＞酰胺态氮(N5)＞生物固氮(N0),其中N0处理的根瘤干重、数量最少,而N2处理的根瘤干重和数量最多.根瘤含氮量的变化为N1＞N4＞N5＞N2＞N3＞N0,其中N1处理下的根瘤中的根瘤含氮量最高,N1、N4和N5各处理与N0处理间差异显著(P＜5%).而氮积累的变化为N2＞N4＞N1＞N5＞N3＞N0,各处理与N0处理相比,差异极显著(P＜1%),与含氮量的变化趋势略有不同.添加不同形态的氮源对根瘤固氮酶活性有抑制作用,其变化趋势为N0＞N5＞N3＞N2＞N4＞N1,N0处理的固氮酶活性最高.
채용광재적배양방법,통과시용불동형태담비,탐토불동형태담대대두근류생장、함담량、담적루량이급고담매활성적영향.결과표명:불동형태적담원,대근류간중、수량유촉진작용,동시증가료근류중적함담량화담적루량.근류간중급수량적변화균위안태담(N2)＞단백담(N4)＞초태담(N1)＞안기산태담(N3)＞선알태담(N5)＞생물고담(N0),기중N0처리적근류간중、수량최소,이N2처리적근류간중화수량최다.근류함담량적변화위N1＞N4＞N5＞N2＞N3＞N0,기중N1처리하적근류중적근류함담량최고,N1、N4화N5각처리여N0처리간차이현저(P＜5%).이담적루적변화위N2＞N4＞N1＞N5＞N3＞N0,각처리여N0처리상비,차이겁현저(P＜1%),여함담량적변화추세략유불동.첨가불동형태적담원대근류고담매활성유억제작용,기변화추세위N0＞N5＞N3＞N2＞N4＞N1,N0처리적고담매활성최고.
Nitrogen plays an important role in nitrogen fixation of soybean. In this paper, a pot culture experiment was carried out to study the effects of different forms nitrogen on the variation of dry weight ( DW) , number, nitrogen content, nitrogen accumulation and nitrogenase activity in nodule of soybean ( Glycine max L. ) at flowering stage. The results showed that different forms nitrogen promoted nodule formation and growth, which embodied in dry weight and number significantly increased compared with biological nitrogen fixation. The order of the nodule DW and nodule number were NO_4~+-N(N2) > Protein-N(N4) >NO_3~-N(N1) > Amino Acid-N(N3 ) >Amide-N(N5) > BiologicalNitrogen Fixation ( NO) , the least nodule DW and number appeared in NO treatment, while the most occurred in N2 treatment. Meanwhile different forms nitrogen increased N content and N accumulation in nodule. The trend of the nitrogen content in nodule was as follows: Nl > N4 > N5 > N2 > N3 > NO, and nitrogen content was the highest in the N1 treatment, moreover, the difference among NO, Nl, N4, N5 was significan at 5% level. However,the order of the N accumulation was N2 > N4 > Nl > N5 > N3 > NO. The different forms nitrogen remarkably affected nitrogenase activity, the trend was NO > N5 > N3 > N2 > N4 > Nl , and the nitrogenase activity was the highest in NO treatment. Therefore,different forms nitrogen had different effects on nitrogen fixation of soybean.