CAJ | 학술논문

以我国363份栽培和野生大豆资源为材料,对大豆胞囊线虫抗性候选基因(rhgl和Rhg4)的SNP位点(8个)进行遗传变异分析,以期阐明野生和栽培大豆间遗传多样性及连锁不平衡水平差异.结果表明,与野生大豆相比,代表我国栽培大豆总体资源多样性的微核心种质及其补充材料的连锁不平衡水平较高(R2值为0.216).在栽培大豆群体内,基因内和基因间分别有100%和16.6%的SNP位点对连锁不平衡显著,形成两个基因特异的连锁不平衡区间(Block).在所有供试材料中共检测到单倍型46个,野生大豆的单倍型数目(27)少于栽培大豆(31),但单倍型多样性(0.916)稍高于栽培大豆(0.816).单倍型大多数(67.4%)为群体所特有(31个),其中15个为野生大豆特有单倍型.野生大豆的两个主要优势单倍型(Hap_10和Hap_11)在栽培大豆中的发生频率也明显下降,推测野生大豆向栽培大豆进化过程中,一方面形成了新的单倍型,另一方面因为瓶颈效应部分单倍型的频率降低甚至消失.
이아국363빈재배화야생대두자원위재료,대대두포낭선충항성후선기인(rhgl화Rhg4)적SNP위점(8개)진행유전변이분석,이기천명야생화재배대두간유전다양성급련쇄불평형수평차이.결과표명,여야생대두상비,대표아국재배대두총체자원다양성적미핵심충질급기보충재료적련쇄불평형수평교고(R2치위0.216).재재배대두군체내,기인내화기인간분별유100%화16.6%적SNP위점대련쇄불평형현저,형성량개기인특이적련쇄불평형구간(Block).재소유공시재료중공검측도단배형46개,야생대두적단배형수목(27)소우재배대두(31),단단배형다양성(0.916)초고우재배대두(0.816).단배형대다수(67.4%)위군체소특유(31개),기중15개위야생대두특유단배형.야생대두적량개주요우세단배형(Hap_10화Hap_11)재재배대두중적발생빈솔야명현하강,추측야생대두향재배대두진화과정중,일방면형성료신적단배형,령일방면인위병경효응부분단배형적빈솔강저심지소실.
For clarifying the diffefence of genetic diversity and linkage disequilibrium(LD)level between cultivated (Glycine max(L.)Merr.)and annual wild soybean(Glycine soja Sieb.& Zucc.),genetic variation pattern of 8 SNP loci developed from soybean cyst nematode resistance candidate genes rhg1 and Rhg4 in soybean germplasm were analyzed.The results indicated that G.max population.consisted of cultivated soybean mini-core collection and modem cultivars,had a higher LD levels(R2 value is 0.216)than G.soja population.Since 100% of pairwise loci within a gene and 16.6% of pairwise loci between genes were significant in G.max population,two specific LD regions were formed for each gene.A total of 46 haplotypes were detected in 363 soybean germplasm.The population of G.soja had less number of haplotypes and higher haplotype diversity than the population of G.max.Among the 31 population-specific haplotypes,15 haplotypes were specific for G.soja population.In addition,the frequency of two major predominant haplotypes(Hap_10 and Hap_11)in G.soja population was obviously decreased in G.max population,which might indicate that some new haplotypes were formed and some old haplotypes were lost during the G.max domesticated from G.soja.