中国法医学杂志
中國法醫學雜誌
중국법의학잡지
CHINESE JOURNAL OF FORENSIC MEDICINE
2009年
6期
372-375
,共4页
方超%汪冠三%胡孙林%梁鸿儒%李英儒%罗思敏%竞花兰
方超%汪冠三%鬍孫林%樑鴻儒%李英儒%囉思敏%競花蘭
방초%왕관삼%호손림%량홍유%리영유%라사민%경화란
法医病理学%时间推断%异物%图像分析
法醫病理學%時間推斷%異物%圖像分析
법의병이학%시간추단%이물%도상분석
forensic pathology%estimating time%foreign body%image analysis
目的 比较不同时间异物多核巨细胞数、细胞核数、Ⅰ/Ⅲ型胶原纤维及Ⅰ型胶原纤维/嗜银纤维比例的变化,推断异物(医用纱布)遗留腹腔时间.方法 采用F344大鼠腹腔箭置医用纱布的方法制作异物遗留腹腔2,8,30,120d的动物模型,肉眼观察大网膜包裹异物程度,HE染色观察异物多核巨细胞数、细胞核数变化,天狼猩红偏振光法观察Ⅰ/Ⅲ型胶原纤维比例变化、银染法规察Ⅰ型胶原纤维/嗜银纤维比例变化,并用图像分析系统对上述结果进行分析.结果 随纱布留置腹腔时间延长,异物多核巨细胞数、细胞核数及Ⅰ/Ⅲ型胶原纤维比例、Ⅰ型胶原纤维/嗜银纤维比例逐渐上升,差异均具有统计学意义(P<0.01).Ⅰ型胶原纤维/嗜银纤维比例与纱布留置腹腔天数之间存在Power函数关系:Days=23.5[Proportion]~(1.6),r=0.972.结论 异物多核巨细胞数、细胞核数、Ⅰ/Ⅲ型胶原纤维及Ⅰ型胶原纤维/嗜银纤维比例变化规律有助于异物(纱布)遗留腹腔时间推断.
目的 比較不同時間異物多覈巨細胞數、細胞覈數、Ⅰ/Ⅲ型膠原纖維及Ⅰ型膠原纖維/嗜銀纖維比例的變化,推斷異物(醫用紗佈)遺留腹腔時間.方法 採用F344大鼠腹腔箭置醫用紗佈的方法製作異物遺留腹腔2,8,30,120d的動物模型,肉眼觀察大網膜包裹異物程度,HE染色觀察異物多覈巨細胞數、細胞覈數變化,天狼猩紅偏振光法觀察Ⅰ/Ⅲ型膠原纖維比例變化、銀染法規察Ⅰ型膠原纖維/嗜銀纖維比例變化,併用圖像分析繫統對上述結果進行分析.結果 隨紗佈留置腹腔時間延長,異物多覈巨細胞數、細胞覈數及Ⅰ/Ⅲ型膠原纖維比例、Ⅰ型膠原纖維/嗜銀纖維比例逐漸上升,差異均具有統計學意義(P<0.01).Ⅰ型膠原纖維/嗜銀纖維比例與紗佈留置腹腔天數之間存在Power函數關繫:Days=23.5[Proportion]~(1.6),r=0.972.結論 異物多覈巨細胞數、細胞覈數、Ⅰ/Ⅲ型膠原纖維及Ⅰ型膠原纖維/嗜銀纖維比例變化規律有助于異物(紗佈)遺留腹腔時間推斷.
목적 비교불동시간이물다핵거세포수、세포핵수、Ⅰ/Ⅲ형효원섬유급Ⅰ형효원섬유/기은섬유비례적변화,추단이물(의용사포)유류복강시간.방법 채용F344대서복강전치의용사포적방법제작이물유류복강2,8,30,120d적동물모형,육안관찰대망막포과이물정도,HE염색관찰이물다핵거세포수、세포핵수변화,천랑성홍편진광법관찰Ⅰ/Ⅲ형효원섬유비례변화、은염법규찰Ⅰ형효원섬유/기은섬유비례변화,병용도상분석계통대상술결과진행분석.결과 수사포류치복강시간연장,이물다핵거세포수、세포핵수급Ⅰ/Ⅲ형효원섬유비례、Ⅰ형효원섬유/기은섬유비례축점상승,차이균구유통계학의의(P<0.01).Ⅰ형효원섬유/기은섬유비례여사포류치복강천수지간존재Power함수관계:Days=23.5[Proportion]~(1.6),r=0.972.결론 이물다핵거세포수、세포핵수、Ⅰ/Ⅲ형효원섬유급Ⅰ형효원섬유/기은섬유비례변화규률유조우이물(사포)유류복강시간추단.
Objective To estimate time of gauze swabs left in abdomen with comparison of the number of foreign body giant cells,theirs nuclei and the proportion of type Ⅰ collagenous fibers to type Ⅲ and argentaffin fibers in different times.Methods F344 rat models dependent 2,8,30,120 days were established by gauze swab fixed in the abdomen,and were studied on gauze swab wrapped by greater omentum,the number of foreign body giant cells and theirs nuclei by HE staining,and the proportion of type Ⅰ collagenous fibers to type Ⅲ and argentaffin fibers by sirius red and silver staining respectively.The results were analyzed by image analysis system.Results The results showed that number of foreign body giant cells,theirs nuclei and the proportion of type Ⅰ collagenous fibers to type Ⅲ and argentaffin fibers increased gradually(P<0.01)followed the time delayed.The Proportion of type Ⅰ collagenous fibers to argentaffin fibersis Power Function of Days of guaze swab left in rat abdomen(r=0.972).Conclusion The number of foreign body giant cells,theirs nuclei and the proportion of type Ⅰ collagenous fibers to type Ⅲ and argentaffin fibers contribute to the estimation of foreign body(eg.gauze swab)left in abdomen.
